LC-MS for glycans, glycopeptides and payload derivatives were performed on HPLC-SQ2 detector (Waters) with a Waters XBridge C18 column (3.5 μm, 2.1× 50 mm) using water containing 0.1% formic acid as phase A, MeCN containing 0.1% formic acid as phase B. The analytical HPLC for payload derivatives was analyzed with an Aglient Eclipse SDB-C18 column (5 μm, 3.0× 250 mm) under UV 214 nm and 280 nm with methods specialized for each compound. HR-ESI-MS was performed with Exactive Plus Orbitrap Mass Spectrometer (Thermo Scientific) equipped with a Waters XBridge C18 column (3.5 μm, 2.1× 50 mm).
Eclipse sdb c18 column
Manufactured by Agilent Technologies
Sourced in United States
The Eclipse SDB-C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of analytes. It features a silica-based stationary phase with a C18 bonded ligand, providing reversed-phase chromatographic separation. This column is suitable for various applications, including pharmaceutical, environmental, and food analysis.
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Lab products found in correlation
2 protocols using eclipse sdb c18 column
1
Glycan, Glycopeptide, and Payload Derivative Analysis
2
HPLC Analysis of Phenolic Acids
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The phenolic acids were analyzed using HPLC (UV detector at 280 nm, EX1600, Exformma, Fairfield, OH, USA) with an Eclipse SDB C18 column (250 mm × 4.6 mm, Agilent, Palo Alto, CA, USA) at 35 °C. Elution was carried out using a linear gradient system consisting of solvent A (water–acetic acid 99.5:0.5) and solvent B (methanol–water–acetic acid 95:0.5:0.5): 0–5 min, 5% B–5% B; 5–10 min, 5% B–25% B; 10–30 min, 25% B–40% B; 30–45 min, 40% B–50% B; 45–55 min, 50% B–100% B; 55–60 min, 100% B–100% B; 60–65 min, 100% B–5% B. All samples were filtered through a 0.45-μm filter before analysis [10 (link)].
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