Inorganic salts and Tris were purchased from MP Biomedicals (France). Bivalirudin trifluoroacetate was purchased from Selleck Chemicals, USA; DNA aptamer HD1, 5′-ggttggtgtggttgg-3′, was synthesized by Evrogene (Russian Federation). Standard platelet-poor human plasma (normal plasma) and all deficient human plasma were purchased from Siemens (Germany). The Technothrombin® TGA Kit, the whole set of reagents for the TGT, was purchased from Technoclone (Austria).
Inorganic salts
Manufactured by MP Biomedicals
Sourced in France
Inorganic salts are chemical compounds that consist of a metal cation and a non-metal anion. They serve as the fundamental building blocks for various laboratory applications and research studies. These salts exhibit a wide range of physical and chemical properties, making them versatile tools in the field of chemistry and allied sciences.
Automatically generated - may contain errors
Lab products found in correlation
Bivalirudin trifluoroacetate, by Selleck Chemicals (2 mentions)
Technothrombin tga kit, by Technoclone (1 mentions)
Standard human plasma, by Siemens (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc hcl, by Carl Roth (1 mentions)
Ethanolamine, by Merck Group (1 mentions)
Human factor xa, by Prolytix (1 mentions)
Human thrombin, by Prolytix (1 mentions)
Milli q, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane tris, by MP Biomedicals (1 mentions)
Sodium salt of n hydroxysulfosuccinimide s nhs, by Chem-Impex (1 mentions)
4 protocols using inorganic salts
1
Anticoagulant Aptamer-Based Thrombin Generation
2
Plasma Coagulation Assays Protocol
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Inorganic salts and Tris were purchased from MP Biomedicals (France). Recombinant human thrombin with a specific activity of 3.6 kIU mg−1, and murine thrombin with a specific activity of 3.8 kIU mg−1 were from HTI, USA; human plasma fibrinogen was from Calbiochem, Germany; bivalirudin trifluoroacetate was from Selleck Chemicals, USA. DNA oligonucleotide RA-36 (GGTTGGTGTGGTTGGTGGTTGGTGTGGTTGG ) was synthesized by ‘APTO-PHARM’ LTD, Russian Federation. Standard human plasma, thrombin time reagent, and prothrombin time reagent for coagulation tests were from Siemens, Germany; murine citrated plasma was purchased from Sigma-Aldrich, USA.
3
Characterization of Thrombin-Fibrinogen Interaction
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Inorganic salts (analytical grade) were purchased from MP Biomedicals (Santa Ana, CA, USA). Recombinant human thrombin with specific activity 3.6 kIU mg À1 (HTI, Essex Junction, VT, USA) and fibrinogen from human plasma (Calbiochem, Schwalbach, Germany) were used. All reactions were carried out at room temperature in a buffer containing 20 mM Tris-acetate, pH 7.4, 0.14 M NaCl, 50 mM KCl, 10 mM MgCl 2 and 10 mM CaCl 2 . The GM modifier for HOPG, (CH 2 ) n (NCH 2 CO) m -NH 2 [30] , was obtained from Nanotuning (Nanotuning, Chernogolovka, Russia).
4
Biomolecular Coupling Protocol
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Inorganic salts and tris(hydroxymethyl)aminomethane (tris) were purchased from MP Biomedicals (Illkirch, France) and Sigma-Aldrich (St. Louis, MO, USA). Human thrombin with a specific activity of 4.2 kIU/mg and human factor Xa were purchased from Haematologic Technologies (Essex Junction, VT, USA). Ethanolamine from Sigma-Aldrich (St. Louis, MO, USA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC-HCl) from Roth (Karlsruhe, Germany), and sodium salt of N-hydroxysulfosuccinimide (s-NHS) from Chem-Impex Int'l (Wood Dale, IL, USA) were used. DNA oligonucleotides were synthesized and purified by polyacrylamide gel electrophoresis by Synthol (Moscow, Russia). Ultra-pure water (18.2 MΩ•cm) was obtained with Milli-Q (Merck KGaA, Darmstadt, Germany) and used to prepare all buffers and solutions.
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