Rat anti ly6g clone 1a8

Manufactured by BD

The Rat anti-Ly6G (clone 1A8) is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Ly6G surface antigen, which is expressed on granulocytes, primarily neutrophils. This antibody can be used to identify and quantify these cell types in various research assays.

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Lab products found in correlation

Anti cd45, by BD (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Rabbit anti s100a4, by Abcam (1 mentions)

Spelling variants of this product

Anti-Ly6G (64 citations) Ly6G (1A8, (44 citations) Ly6G (clone 1A8, (21 citations) Anti-Ly6G (clone 1A8, (17 citations) Anti-Ly6G (1A8, (16 citations) Rat anti-Ly6G (9 citations) Clone 1A8 (4 citations) Purified Rat anti-mouse Ly6G (clone 1A8) (3 citations)

2 protocols using rat anti ly6g clone 1a8

Antibody Immunoblotting Protocol

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Antibodies were as following: goat anti-Siglec-E (R&D Systems, Minneapolis, MN), rabbit anti-Gstp1 (Sigma–Aldrich, St. Louis, MO), mouse anti-β-actin (Sigma–Aldrich), rabbit anti-β-actin (Cell Signaling, Danvers, MA), anti-CD45 (BD Pharmingen, San Jose, CA), rat anti-Ly6G (clone 1A8, BD Pharmingen), mouse anti-Gstp1 (BD Pharmingen), and rabbit anti-β-galactosidase (Bioss Antibodies, Woburn, MA). Secondary antibodies were from LI-COR (Lincoln, NE) or Jackson ImmunoResearch Laboratories (West Grove, PA).

Schwarz F., Pearce O.M., Wang X., Samraj A.N., Läubli H., Garcia J.O., Lin H., Fu X., Garcia-Bingman A., Secrest P., Romanoski C.E., Heyser C., Glass C.K., Hazen S.L., Varki N., Varki A, & Gagneux P. (2015). Siglec receptors impact mammalian lifespan by modulating oxidative stress. eLife, 4, e06184.

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Immunostaining for CD3, Ly6G, and S100A4

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Immunostaining was performed as described previously [53] (link) using the following primary antibodies: rat anti-CD3 (clone 17A2, BD Biosciences, 1∶500), rat anti-Ly6G (clone 1A8, BD Biosciences, 1∶5000) and rabbit anti-S100A4 (Abcam, 1∶200).

Dumas A., Amiable N., de Rivero Vaccari J.P., Chae J.J., Keane R.W., Lacroix S, & Vallières L. (2014). The Inflammasome Pyrin Contributes to Pertussis Toxin-Induced IL-1β Synthesis, Neutrophil Intravascular Crawling and Autoimmune Encephalomyelitis. PLoS Pathogens, 10(5), e1004150.

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