Ab109211

Antibodies against Ki67 (ab16667, Abcam, 1:200), α-Sarcomeric Actinin (SA, ab9465, Abcam, 1:200), vWF (ab6994, Abcam, 1:100), CD31 (ab28364, Abcam, 1:100), Podoplanin (ab10288, Abcam, 1:500), Vimentin (ab92547, Abcam, 1:500), Sca-1 (ab109211, Abcam, 1:200), α-SMA (ab32575, Abcam, 1:500), MPO (PA5-16672, Thermo Fisher, 1:100), CD4 (ab237722, Abcam, 1:200), CD8 (ab33786, Abcam, 1:100), cTnT (MS-295P, Invitrogen, 1:100), Nkx2.5 (ab106923, Abcam, 1:100) as well as Alexa Fluo 594 or 488 conjugated Goat anti Rabbit or mouse secondary antibodies (1:500) were purchased from Abcam. TUNEL staining kit was purchased from Promega (G3250).

Lung tissues were lysed in RIPA lysis buffer (Beyotime, China) to obtain total proteins. The protein concentration was determined by a BCA protein assay kit (Thermo Fisher Scientific, USA). Equal amounts of proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes. After being blocked for 1 h, PVDF membranes were incubated with specific primary antibodies against PU.1 (sc-390405, Santa Cruz Biotechnology, USA), Sca-1 (ab109211, Abcam, UK), β-catenin (8480, Cell Signaling Technology, USA), and β-tubulin (10068-1-AP, Proteintech, China) overnight at 4^oC. Then, these membranes were incubated with anti-mouse or anti-rabbit IgG HRP-labeled secondary antibody (Proteintech, China) for one hour at room temperature. Labeled proteins were detected by the ECL plus Western blotting detection system (Bio-Rad, USA).