Pdf gal80

Fly stocks used for this study are as follows: wild-type Drosophila melanogaster Canton-S (CS), ap::GFP [Bloomington stock center (BS), #38423], Pdf-GAL4 (BS, #6900), nSyb-GAL4 (BS, #51941), c929 (BS, #25373), Mai179 (obtained from Dr. Orie T. Shafer, University of Michigan), Pdf-GAL80 (obtained from Leslie C. Griffith, Brandeis University), UAS-ap RNAi (NIG-fly, 8376R-1), UAS-ap^ΔHD (see next section), UAS-ap^ΔLIM (see next section), UAS-Chi RNAi (VDRC, 43934), UAS-Chi^ΔLID (obtained from Dr. Veronica Rodriguez, Tata Institute of Fundamental Research), UAS-Chi^ΔDD (obtained from Dr. Veronica Rodriguez), UAS-dORK1ΔNC (BS, #6587), UAS-mCherry.NLS (BS, #38424), UAS-IVS-mCD8::RFP (BS, #32218), and UAS-GFP RNAi (NIG-fly, GFP-IR-2). Flies were raised on glucose-yeast-cornmeal medium at 25.0 ± 0.5 °C in a 12-h light:12-h dark (LD) cycle. All lines except for UAS-mCherry.NLS and UAS-IVS-mCD8::RFP were outcrossed for at least six generations to white flies with the CS genetic background.

Flies were maintained on a media of sucrose, yeast, molasses, and agar under 12:12 LD cycles at 25 °C. One- to 3-day-old female flies were separated and maintained on standard cornmeal-yeast medium under 12:12 LD cycles at 25 °C for 4 nights before experiments began. Clk[out] (56754), per^s (80919), pdf-Gal4 (6899), pBDP (pBDP-Gal4Uw)(68384), pBDP split (p65-AD Uw; Gal4-DBD Uw) (79603), R23E10-Gal4 (49032), R69F08-Gal4 (39499), R58H05 p59AD (70750), R48H04 DBD (69353) pdf-Gal80 (80940), R51H05 p65AD (70720), R18H11 DBD (69017), R92H07-Gal4 (40633), R52B02-Gal4 (38814), R20D01-Gal4 (48889), BRPstar (55751),UAS-GCaMP6s (42746), UAS-TNT (28838), UAS-kir2.1 (6596) and UAS-hid (65403) were obtained from the Bloomington Drosophila Stock Center. mGluR-RNAi (1793) was obtained from Vienna Drosophila Resource Center. MB122B and 20xUas-IVS-Syn-GFP was obtained from Janelia Farm.