Macrophages were treated with LPS (from 0 μg/mL to 60 μg/mL) for 24 h. After being washed twice with phosphate buffer saline, cells were incubated with FITC-conjugated-anti-CD97 (BD, USA). Then, analysis was performed using a flow cytometer (Beckman, USA) with cell Quest Pro Software.
Fitc conjugated anti cd97
Manufactured by BD
Sourced in United States
FITC-conjugated-anti-CD97 is a fluorescently labeled antibody that binds to the CD97 antigen. CD97 is a G protein-coupled receptor expressed on the surface of various cell types. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and identification of cells expressing CD97.
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Lab products found in correlation
2 protocols using fitc conjugated anti cd97
1
CD97 Expression in LPS-Treated Macrophages
2
Flow Cytometric Analysis of CD97
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Following treatment, the cells were washed twice with PBS and then incubated with FITC-conjugated anti-CD97 (BD Biosciences, Franklin Lakes, NJ, USA). The subsequent analysis of the expression of CD97 was performed using a flow cytometer (BD Biosciences).
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