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Amp15n5

Manufactured by Merck Group
Sourced in United States, United Kingdom

The AMP15N5 is a high-performance laboratory equipment designed for various analytical applications. It is a precision instrument that delivers reliable and accurate measurements. The core function of the AMP15N5 is to provide users with the necessary tools to conduct their research and analyses effectively. No further details or interpretations are provided.

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2 protocols using amp15n5

1

Analytical Standards for Nucleotides

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Analytical standards of adenine (Ade), adenosine 5′-diphosphate (ADP) and adenosine 5′-triphosphate (ATP) were obtained from Bio Basic Inc. (Toronto, Canada); adenosine 5′-monophosphate (AMP) was obtained from MP Biomedicals™, (State, USA). Isotope-labelled internal standard AMP15N5 was purchased from Sigma–Aldrich, (St., USA).
Ultrapure water (Milli-Q) was produced by a Millipore system (France). Acetic acid and citric acid were obtained from Sigma–Aldrich, St. Louis, USA. Ammonia water (28%) was acquired from Nihon Shiyaku Reagent, Japan. The three kinds of sorbents used in dispersive solid phase extraction (dSPE) step are listed below: Primary and secondary amine (PSA) SPE bulk sorbent (Agilent Technologies, USA), C18 Endcapped SPE bulk sorbent (C18EC), 57.9 μm, 59 A (Agilent Technologies, USA), Graphitized Carbon Black (GCB) (Waters, Ireland). The Oasis® MAX Cartridge used in solid phase extraction (SPE), 30 μm (Waters, USA).
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2

HILIC Analysis of Metabolite Standards

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All solvents and mobile phase additives were LC-MS-grade. Acetonitrile (CHROMASOLV LC-MS) was purchased from Scientific Laboratory Supplies (Wilford, UK), methanol and water were purchased from Fisher Scientific (Leicestershire, UK), ammonium bicarbonate was obtained from Fluorochem (Hadfield, UK), and ammonium hydroxide was from Sigma-Aldrich (Gillingham, UK). All authentic chemical standards used for the annotation of metabolites were of analytical or higher grade and were obtained from various suppliers (Sigma-Aldrich (Gillingham, UK), VWR (Leicestershire, UK), and Fisher Scientific (Leicestershire, UK)). They were accurately weighed and dissolved in appropriate volumes of LC-MS-grade water to obtain individual stock solutions at concentrations of 1 mg/mL. Appropriate amounts of each stock solution were mixed to obtain four standard mixtures at concentrations of 25 µg/mL in water that were further prepared for HILIC analysis, as described below. Stable isotope-labelled internal standards AMP-15N5, ADP-15N5, and ATP-13C10 were purchased from Sigma-Aldrich (Gillingham, UK) and prepared in a mixture at a concentration of 48 µg/mL.
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