For the characterization of XPC expression, both cell lines were fixed with 4% paraformaldehyde followed by permeabilization using 0.2% Triton X-100 and saturation with 3% FBS in PBS. Primary antibody targeting XPC (mouse monoclonal, Thermofisher Scientific, Waltham, MA, USA) was utilized followed by incubation with Alexa Fluor 488-coupled secondary antibody (goat anti-mouse, Invitrogen, Waltham, MA, USA). Nuclear DNA was counter-stained with Hoechst (Sigma-Aldrich, Burlington, MA, USA). Cell images were acquired by the Zen Axio-observer at 40X.
Alexa fluor 488 coupled secondary antibody goat anti mouse
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 488-coupled secondary antibody (goat anti-mouse) is a fluorescently labeled antibody that binds to mouse primary antibodies. It is used as a detection reagent in various immunoassay techniques.
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Lab products found in correlation
2 protocols using alexa fluor 488 coupled secondary antibody goat anti mouse
1
Quantitative XPC Protein Immunodetection
2
XPC Expression Characterization Protocol
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For the characterization of XPC expression, both cell lines were xed with 4% paraformaldehyde followed by permeabilization using 0.2% Triton X-100 and saturation with 3% FBS in PBS. Primary antibody targeting XPC (mouse monoclonal, Thermo sher Scienti c) was utilized followed by incubation with Alexa Fluor 488-coupled secondary antibody (goat anti-mouse, Invitrogen). Nuclear DNA was counterstained with Hoechst (Sigma-Aldrich). Cell images were acquired by the Zen Axio-observer at 40X.
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