Twenty-five micrometer frozen sections of tumors were processed with anti-Ki67 antibody (#12075, Cell Signaling, Danvers, MA, USA), followed by Taxes Red-conjugated IgG (#TI-1000, Vector Laboratories, Burlingame, CA, USA) and Lectin (Galectin, Alexa Flour 488 conjugated, #FLK-2100, Vector Laboratories, Burlingame, CA, USA). Sections were visualized using an Olympus Fluoview FV1000 confocal microscope (Olympus, Japan) and processed using ImageJ 1,54b software (http://imagej.nih.gov/ij , version 1.54b, assessed on 8 January 2023).
Taxes red conjugated igg
Manufactured by Vector Laboratories
Sourced in United States
Alexa-conjugated IgG is a fluorescently-labeled secondary antibody product offered by Vector Laboratories. It is designed for use in immunodetection and other fluorescence-based applications. The Alexa Fluor 647 dye is covalently attached to purified IgG, providing a bright, photostable label for target detection.
Automatically generated - may contain errors
2 protocols using taxes red conjugated igg
1
Tumor Proliferation Quantification
2
Quantifying Tumor Cell Proliferation and Apoptosis
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Twenty-five micrometer frozen sections of tumors were processed with anti-Ki67 antibody (#12075, Cell Signaling, Danvers, MA, USA), followed by Taxes Red-conjugated IgG (#TI-1000, Vector Laboratories, Burlingame, CA, USA). A TUNEL assay kit (#ab206386, Abcam, Boston, MA, USA) was used according to the manufacturer’s protocol. Sections were visualized using an Olympus Fluoview FV1000 confocal microscope (Olympus, Japan) and processed using ImageJ software (http://imagej.nih.gov/ij , version 1.54b, assessed on 08 January 2023).
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