APP23 mice were treated with SUS+MB and were perfused with PBS and drop fixed in 4% paraformaldehyde in PBS. They were then cryoprotected in 30% sucrose in PBS and sectioned at 40 μm with a freezing sliding microtome. Microglia were immunostained with anti‐Iba1 antibody (Wako, JP 1:1000) followed by incubation with an anti‐rabbit secondary antibody AlexaFluor 568 conjugate (Invitrogen). Sections were then co‐stained with 10 μM methoxy‐XO4 (Tocris Bioscience) in PBS with 20% ethanol before cover‐slipping. Images were obtained with a spinning disk confocal microscope (Nikon Diskovery) with a 20× objective, acquiring z‐stacks through the entire depth of the section (Figure 1d ).
Diskovery
Manufactured by Nikon
The Diskovery is a laboratory equipment product designed for scientific applications. It serves as a high-precision disk mill for grinding and homogenizing solid samples. The core function of the Diskovery is to efficiently prepare samples for further analysis or processing.
Automatically generated - may contain errors
2 protocols using diskovery
1
Microglial Morphology Analysis in APP23 Mice
2
Microglia Visualization in APP23 Mice
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APP23 mice were treated with SUS +MB and were perfused with PBS and drop fixed in 4% paraformaldehyde in PBS. They were then cryoprotected in 30% sucrose in PBS and sectioned at 40 µm with a freezing sliding microtome. Microglia were immunostained with anti-Iba1 antibody (Wako, JP 1:1,000) followed by incubation with an anti-rabbit secondary antibody AlexaFluor 568 conjugate (Invitrogen). Sections were then co-stained with 10 μM methoxy-XO4 (Tocris Bioscience) in PBS with 20% ethanol before cover-slipping. Images were obtained with a spinning disk confocal microscope (Nikon Diskovery) with a 20x objective, acquiring z-stacks through the entire depth of the section (Fig 1D).
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