Previously established PDAC patient-derived xenografts (PDAC PDX) were obtained from Dr Manuel Hidalgo under a Material Transfer Agreement with the Centro Nacional de Investigaciones Oncológicas (CNIO) (Reference no. I409181220BSMH). The indicated PDXs were expanded in female 6–8 week-old NU-Foxn1nu nude mice (Envigo, Spain) as previously described.30 (link) PDXs 110314, 140114 and 010414 originated from primary patient PDAC tumours resected at Rechts der Isar Hospital, Technical University of Munich and subsequently established at the CNIO.
Nu foxn1nu nude mice
Manufactured by Inotiv
Sourced in United States
The NU-Foxn1nu nude mice are a strain of immunodeficient mice. They are characterized by the absence of a functional thymus gland, resulting in a lack of mature T cells. This strain is commonly used in research applications where the study of human tumor xenografts or other transplanted tissues is required.
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Lab products found in correlation
2 protocols using nu foxn1nu nude mice
1
PDAC Patient-Derived Xenograft Expansion
2
Xenografting Pancreatic Tumor Cells in Mice
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Female 6- to 8-week-old NU-Foxn1nu nude mice (Envigo, Spain) were subcutaneously injected with 10,000, 1000, or 500 control or PP2-pretreated PDAC Panc354 cells in 50 µL of MatrigelTM (Cat no. 734-0270, Corning, Corning, NY, USA). Tumor growth was monitored biweekly for up to 10 weeks. If a mouse in a specific dilution group warranted sacrifice (e.g., ulcerated tumor), all of the mice (Control and Treated) in that dilution group were sacrificed in order to obtain the total number of tumors for all mice at the exact same time. Mice were sacrificed at 4, 8, and 10 weeks post-inoculation for the 10,000-, 1000-, and 500-cell dilutions, respectively.
Mice were housed according to institutional guidelines, and all experiments were performed in compliance with the institutional guidelines for the welfare of experimental animals approved by the Universidad Autónoma de Madrid Ethics Committee (CEI 60-1057-A068) or by the Instituto de Salud Carlos III Ethics Committee (CBA12_2014-v3) and La Comunidad de Madrid (PROEX 335/14 or PROEX 53/14) and in accordance with the guidelines for Ethical Conduct in the Care and Use of Animals, as stated in The International Guiding Principles for Biomedical Research involving Animals, developed by the Council for International Organizations of Medical Sciences (CIOMS).
Mice were housed according to institutional guidelines, and all experiments were performed in compliance with the institutional guidelines for the welfare of experimental animals approved by the Universidad Autónoma de Madrid Ethics Committee (CEI 60-1057-A068) or by the Instituto de Salud Carlos III Ethics Committee (CBA12_2014-v3) and La Comunidad de Madrid (PROEX 335/14 or PROEX 53/14) and in accordance with the guidelines for Ethical Conduct in the Care and Use of Animals, as stated in The International Guiding Principles for Biomedical Research involving Animals, developed by the Council for International Organizations of Medical Sciences (CIOMS).
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