Cresyl violet (Sigma) or LysoTracker DND-99 (Thermo Fisher) was used to label acidic lysosomes. For fluorescence analysis of sorted subsets or BMDM, cells were incubated with 5 μM Cresyl violet or 200 nM LysoTracker DND-99 for 1 h at 37°C. Cells were washed 2 times and fixed in 1% PFA overnight at 4°C. Images were taken using an in-house Leica fluorescent microscope or a CSU-22 Spinning Disk Confocal. For flow cytometry, cells were incubated with 2 μM Cresyl violet for 30 min at 37°C and processed as described in the cathepsin activity assays.
Csu 22 spinning disk confocal
Manufactured by Leica
The CSU-22 Spinning Disk Confocal is a high-speed confocal imaging system designed for live-cell imaging. It utilizes a unique dual-rotating disk design to provide optical sectioning and high-speed image acquisition. The system is capable of capturing images at up to 2,000 frames per second, making it suitable for a wide range of live-cell imaging applications.
Automatically generated - may contain errors
3 protocols using csu 22 spinning disk confocal
1
Lysosomal Staining and Analysis
2
Lysosomal Staining of Immune Cells
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Cresyl violet (Sigma) or LysoTracker DND-99 (Thermo Fisher) was used to label acidic lysosomes. For fluorescence analysis of sorted subsets or BMDM, cells were incubated with 5 μM Cresyl violet or 200 nM LysoTracker DND-99 for 1 h at 37 °C. Cells were washed 2 times and fixed in 1% PFA overnight at 4 °C. Images were taken using an in-house Leica fluorescent microscope or a CSU-22 Spinning Disk Confocal. For flow cytometry, cells were incubated with 2 μM Cresyl violet for 30 min at 37 °C and processed as described in the cathepsin activity assays.
3
Lysosome Labeling for Cellular Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cresyl violet (Sigma) or LysoTracker DND-99 (Thermo Fisher) was used to label acidic lysosomes. For fluorescence analysis of sorted subsets or BMDM, cells were incubated with 5 μM Cresyl violet or 200 nM LysoTracker DND-99 for 1 h at 37°C. Cells were washed 2 times and fixed in 1% PFA overnight at 4°C. Images were taken using an in-house Leica fluorescent microscope or a CSU-22 Spinning Disk Confocal. For flow cytometry, cells were incubated with 2 μM Cresyl violet for 30 min at 37°C and processed as described in the cathepsin activity assays.
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