Human st2 il 33r duoset elisa

Serum IL-33 and ST2 were detected by enzyme-linked immunosorbent assay (ELISA), and the serum concentrations of IL-33 and ST2 were repeatedly determined using a commercial kit Human ST2/IL-33R DuoSet ELISA (R&D Systems; Minneapolis, MN, USA). All operations were strictly performed in accordance with the instructions.

Serum levels of sST2 and CTGF were analyzed with enzyme-linked immunosorbent assay (ELISA) using the human ST2/IL-33R DuoSet ELISA by R&D Systems (Cat. No: DY523B-05; Minneapolis, MN, USA) and CTGF/CCN2 DuoSet by R&D Systems (Cat. No: DY9190-05; Minneapolis, MN, USA), respectively, according to the manufacturer’s instructions. Activin A serum levels were assessed with an ELISA from RayBiotech (Cat. No: ELH-ActivinA-5, Norcross, GA, USA). sST2, CTGF, and activin A were expressed in ng/mL. Serum serotonin levels were determined by a liquid chromatography tandem mass spectrometry (LC-MS/MS) based assay [23 (link)]. Platelet (plt) counts were determined routinely for clinical practice simultaneous to serotonin measurement, and serotonin was expressed as nmol/10⁹plt. Serum levels of NT-proBNP were determined in serum by an electrochemiluminescence immunoassay used on the Modular Analytics E170 (Roche Diagnostics, Mannheim, Germany) and expressed in pmol/L [24 (link)].

Plasma concentrations of IL-33, ST2IL1RL1, and IL1RAcP were measured by enzyme-linked immunosorbent assay (ELISA) using Human ST2/IL-33R DuoSet ELISA, Human IL-1 RAcP/IL-1 R3 DuoSet ELISA and Human IL-33 Quantikine ELISA Kit (R&D systems, Abingdon, U.K), according to the manufacturer’s protocol. The assay ranges for IL-33, ST2/IL1RL1, and IL1RAcP were 3.1–200 pg/mL, 31.2–2000 pg/mL, and 31.2–2000 pg/mL, respectively.