Prepit l2p extraction kit

DNA was extracted from unaffected and affected samples using tissue‐appropriate protocols. Genomic DNA from peripheral blood lymphocytes was isolated using one of the following: the whole blood isolation protocol and reagents as described in the Gentra Puregene Handbook (Qiagen), the QIAamp DNA Blood Mini Isolation kit (Qiagen), or the Chemagen DNA Blood Kit and MSM I instrument (PerkinElmer). Genomic DNA from tissue biopsies was isolated following the “DNA Purification from Tissues materials” protocol for the QIAamp DNA Mini and Blood Mini Kit (Qiagen). Saliva samples were collected using the Oragene collection kit (DNA Genotek), and DNA was isolated using the prepIT‐L2P extraction kit (DNA Genotek). DNA from buccal samples was isolated using the Xtreme DNA Kit (Isohelix). DNA from cultured amniocytes was isolated using the Gentra Puregene Handbook (Qiagen). DNA from direct amniocytes was isolated using the QIAamp DNA Micro Kit and protocol (Qiagen). Extracted DNA was quantified using the Infinite M200 NanoQuant (Tecan).