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Cd16 af647 clone 3g8

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CD16-AF647 (clone 3G8) is a fluorescently labeled antibody that binds to the CD16 antigen. CD16, also known as FcγRIII, is a low-affinity receptor for the Fc portion of IgG antibodies and is expressed on the surface of natural killer cells, monocytes, and neutrophils. This product can be used to identify and study cells expressing CD16 using flow cytometry or other relevant applications.

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Lab products found in correlation

Cd45 bv421, by BioLegend (2 mentions) Cd45 bv421 clone hi30, by BioLegend (2 mentions) Cd14 af488 clone m5e2, by BioLegend (2 mentions) Cd56 percp cy5.5 clone 5 1h11, by BioLegend (2 mentions) Cd3 af647 clone hit3a, by BioLegend (2 mentions) Cd19 percp clone hib19, by BioLegend (2 mentions) Pan flavivirus antibody clone d1 4g2 4 15, by Merck Group (2 mentions) Lsr 3, by BD (2 mentions) Cd14 af488, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions)

2 protocols using cd16 af647 clone 3g8

1

PBMC Immunophenotyping and ZIKV Infection

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Surface staining of PBMCs were performed with the following antibodies: CD45-BV421 (clone HI30, Biolegend), CD14-AF488 (clone M5E2, Biolegend), CD56-PerCP-Cy5.5 (clone 5.1H11, Biolegend), CD3-AF647 (clone HIT3a, Biolegend) and CD19-PerCP (clone HIB19, Biolegend). PBMCs specimens were either sorted based on surface markers expression using BD LSR III (BD) or proceed on to intracellular ZIKV staining for detection of ZIKV-infected cells. Indirect intracellular ZIKV staining was performed using pan flavivirus antibody (clone D1-4G2-4-15, EMD Millipore), followed by fluorescence-conjugated secondary antibody. For profiling of monocyte subsets, separate surface staining for CD45-BV421, CD14-AF488 and CD16-AF647 (clone 3G8, Biolegend) were performed on PBMCs. All fluorescence-conjugated antibodies were purchased from Biolegend. FACS acquisitions were performed on BD FACSCanto II (BD), using BD FACSDIVA software. All FACS data was analyzed using FlowJo software.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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2

PBMC Immunophenotyping and ZIKV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface staining of PBMCs were performed with the following antibodies: CD45-BV421 (clone HI30, Biolegend), CD14-AF488 (clone M5E2, Biolegend), CD56-PerCP-Cy5.5 (clone 5.1H11, Biolegend), CD3-AF647 (clone HIT3a, Biolegend) and CD19-PerCP (clone HIB19, Biolegend). PBMCs specimens were either sorted based on surface markers expression using BD LSR III (BD) or proceed on to intracellular ZIKV staining for detection of ZIKV-infected cells. Indirect intracellular ZIKV staining was performed using pan flavivirus antibody (clone D1-4G2-4-15, EMD Millipore), followed by fluorescence-conjugated secondary antibody. For profiling of monocyte subsets, separate surface staining for CD45-BV421, CD14-AF488 and CD16-AF647 (clone 3G8, Biolegend) were performed on PBMCs. All fluorescence-conjugated antibodies were purchased from Biolegend. FACS acquisitions were performed on BD FACSCanto II (BD), using BD FACSDIVA software. All FACS data was analyzed using FlowJo software.
Foo S.S., Chen W., Chan Y., Bowman J.W., Chang L.C., Choi Y., Yoo J.S., Ge J., Cheng G., Bonnin A., Nielsen-Saines K., Brasil P, & Jung J.U. (2017). Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nature microbiology, 2(11), 1558-1570.
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