Mouse monoclonal antibodies used in this study included anti-GFP (JL-8; Takara Bio Inc.), anti-ATG14 (4H8; Medical and Biological Laboratories), anti-ATG3 (3E8; Medical and Biological Laboratories), antimonomeric Keima-red (2F7; Medical and Biological Laboratories), anti-Drp1 (clone 8/DLP1; BD), anti-LC3 (clone LC3.1703; Cosmobio), anti-Mfn2 (XX-1; Santa Cruz Biotechnology, Inc.), anti-STX17 (2F8; Medical and Biological Laboratories), anti-Pgk1 (22C5; Molecular Probes), and anti-actin (MAB1501; EMD Millipore). Rabbit polyclonal antibodies that were used included anti-ATG5 (PM050; Medical and Biological Laboratories), anti-Mfn1 (H-65; Santa Cruz Biotechnology, Inc.), and anti-Tom20 (F-145; Santa Cruz Biotechnology, Inc.). Anti-FIP200 rabbit monoclonal antibody (D10D11; Cell Signaling Technology) was used.
Anti mfn1 h 65
Manufactured by Santa Cruz Biotechnology
Anti-Mfn1 (H-65) is a primary antibody product developed by Santa Cruz Biotechnology. It is designed to recognize the Mfn1 (mitofusin 1) protein, which is involved in mitochondrial fusion.
Automatically generated - may contain errors
2 protocols using anti mfn1 h 65
1
Mouse Monoclonal Antibodies for Autophagy and Mitochondrial Dynamics
2
Western Blot Antibody Validation Protocol
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Primary antibodies used in western blot were (Ab code and dilution in parenthesis): rabbit monoclonal anti-Beclin1 (D40C5) (1:1000, 3% BSA in 0.1% TBS-T, Cell Signaling Technology); rabbit polyclonal anti-Bax (Δ 21) (SC6236) (1:1000, 1% BSA in 0.1% TBS-T, Santa Cruz Biotechnology); rabbit polyclonal anti-Bak (06–536) (1:1000, 1% BSA in 0.1% TBS-T, Merck-Millipore); rabbit polyclonal anti-Bcl2 (SAB4500003) (1:1000, 1% BSA in 0.1% TBS-T, Sigma-Aldrich); rabbit polyclonal anti-phospho-c-Myc (M8433) (pThr58/pSer62) (1 μg/ml, 1% BSA in 0.1% TBS-T, Sigma-Aldrich); chicken polyclonal anti-c-Myc (GW21184P) (1:5000, 1% BSA in 0.1% TBS-T, Sigma-Aldrich); rabbit polyclonal anti-Mfn1 (H65) (1:5Z00, 5% non-fat dry milk in 0.1% TBS-T, Santa Cruz Biotechnology).
Secondary antibodies used were: goat anti-mouse IgG-HRP conjugated (SC2005) (1:3000, 1% BSA in 0.1% TBS-T, Santa Cruz Biotechnology); goat anti-chicken IgY-HRP conjugated (SC2428) (1:3000, 1% BSA in 0.1% TBS-T, Santa Cruz Biotechnology); goat anti-rabbit IgG-HRP conjugated (A0545) (1:60 000, 1% BSA in 0.1% TBS-T, Sigma-Aldrich).
Secondary antibodies used were: goat anti-mouse IgG-HRP conjugated (SC2005) (1:3000, 1% BSA in 0.1% TBS-T, Santa Cruz Biotechnology); goat anti-chicken IgY-HRP conjugated (SC2428) (1:3000, 1% BSA in 0.1% TBS-T, Santa Cruz Biotechnology); goat anti-rabbit IgG-HRP conjugated (A0545) (1:60 000, 1% BSA in 0.1% TBS-T, Sigma-Aldrich).
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