Dulbecco’s modified Eagle Medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were obtained from Lonza (Walkersville, MD, USA). 4-[3-(4iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) was obtained from Roche (Cascade, CA, USA).
Freeze-dried powder (organic acai berry powder 99.9% and citric acid 0.1%) of acai berry (Sambazon, Paragon laboratories, Torrance, CA, USA) was purchased from Korean agency. ABWE was prepared by depositing 100 g of acai berry powder in 1 L of water of 80°C and extracted for 3 hr. It was then filtered and freeze-dried for 72 hr. These extraction processes were repeated three times to obtain ABWE for the present study.
Freeze-dried powder (organic acai berry powder 99.9% and citric acid 0.1%) of acai berry (Sambazon, Paragon laboratories, Torrance, CA, USA) was purchased from Korean agency. ABWE was prepared by depositing 100 g of acai berry powder in 1 L of water of 80°C and extracted for 3 hr. It was then filtered and freeze-dried for 72 hr. These extraction processes were repeated three times to obtain ABWE for the present study.