E14 mouse embryonic stem cells (ESCs) were grown in two different media: 'serum+LIF' consisted in Glasgow medium (Sigma), 15% FBS (Gibco), 2mM L-Glutamine, 0.1mM MEM non-essential amino acids (Gibco), 1mM sodium pyruvate (Gibco), 0.1mM bmercaptoethanol, 1000 U/mL leukemia inhibitory factor (LIF, Miltenyi Biotec); '2i+LIF' was made of 50% Neurobasal medium (Gibco), 50% DMEM/F12 (Gibco), 2mM L-Glutamine (Gibco), 0.1mM β-mercaptoethanol, Ndiff Neuro-2 medium supplement (Millipore), B-27 medium supplement (Gibco), 1000 U/mL LIF, 3μM Gsk3 inhibitor (CT-99021), 1μM MEK inhibitor (PD0325901). Cells were cultured in 0.2% gelatin-coated flasks at 37 °C with 5% CO2. Except for the CRISPR-Cas9 loss-of-function screens that were performed in serumbased medium, all experiments were performed in 2i medium.
Gsk3 inhibitor
Manufactured by Thermo Fisher Scientific
The Gsk3 inhibitor is a laboratory reagent used in scientific research. It is a chemical compound that acts as an inhibitor of the glycogen synthase kinase 3 (GSK3) enzyme, which plays a crucial role in various cellular processes. The Gsk3 inhibitor is commonly employed in cell-based assays and in vitro studies to investigate the effects of GSK3 inhibition on cellular function and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Ndiff neuro 2 medium supplement, by Merck Group (2 mentions)
B 27 medium supplement, by Thermo Fisher Scientific (2 mentions)
Glasgow medium, by Merck Group (2 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Neurobasal medium, by Thermo Fisher Scientific (2 mentions)
2 protocols using gsk3 inhibitor
1
Culturing mouse embryonic stem cells
2
Culturing mouse embryonic stem cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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E14 mouse embryonic stem cells (ESCs) were grown in two different media: 'serum+LIF' consisted in Glasgow medium (Sigma), 15% FBS (Gibco), 2mM L-Glutamine, 0.1mM MEM non-essential amino acids (Gibco), 1mM sodium pyruvate (Gibco), 0.1mM bmercaptoethanol, 1000 U/mL leukemia inhibitory factor (LIF, Miltenyi Biotec); '2i+LIF' was made of 50% Neurobasal medium (Gibco), 50% DMEM/F12 (Gibco), 2mM L-Glutamine (Gibco), 0.1mM β-mercaptoethanol, Ndiff Neuro-2 medium supplement (Millipore), B-27 medium supplement (Gibco), 1000 U/mL LIF, 3μM Gsk3 inhibitor (CT-99021), 1μM MEK inhibitor (PD0325901). Cells were cultured in 0.2% gelatin-coated flasks at 37 °C with 5% CO2. Except for the CRISPR-Cas9 loss-of-function screens that were performed in serumbased medium, all experiments were performed in 2i medium.
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