The cross sections of the silicone molds with templated topography and the biofabricated objects were imaged by using an Olympus SZX10 optical microscope with a DP74 camera. The images from the biofilms were taken by placing the biofabricated objects on a Petri dish with DI water.
When two or more images were needed to represent the whole sample, they were stitched by using the Adobe Photoshop CC 2018 software. The replicated height of the features was measured using the ImageJ 2.0.0-rc56/1.51h software. In short, an image of an object of ca. 30 mm height and 320 μm feature width was binarized by using the Otsu automatic thresholding method. Then, the height of the features was manually measured and plotted against the corresponding depth, i.e., the distance relative to the top surface of the biofabricated object.
When two or more images were needed to represent the whole sample, they were stitched by using the Adobe Photoshop CC 2018 software. The replicated height of the features was measured using the ImageJ 2.0.0-rc56/1.51h software. In short, an image of an object of ca. 30 mm height and 320 μm feature width was binarized by using the Otsu automatic thresholding method. Then, the height of the features was manually measured and plotted against the corresponding depth, i.e., the distance relative to the top surface of the biofabricated object.