Citraconate

Manufactured by Merck Group

Citraconate is a laboratory reagent used in various chemical and biological applications. It serves as a versatile building block for organic synthesis and as a precursor for the production of other compounds. The core function of Citraconate is to provide a stable, purified source of the citraconate ion, which can be utilized in a range of research and development activities.

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Lab products found in correlation

2 protocols using citraconate

Optimized Enzyme Activity Assays

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Medium and buffer components were purchased from Fisher Chemical. Amino acids, casAmino acids (acid-hydrolyzed), copper sulfate, ferrous ammonium sulfate, cytochrome c, horseradish peroxidase (HRP), Amplex Red, hydrogen peroxide solution (30% w/w), bovine catalase, ascorbic acid, ovalbumin, β-mercaptoethanol, N-acetylcysteine, bovine superoxide dismutase, reduced (GSH) and oxidized (GSSG) glutathione, E. coli alkaline phosphatase (catalog P5931), ascorbate, p-nitrophenylphosphate, zinc(II) diacetate, guanidinium HCl, citraconate, diethylenetriaminepentaacetic acid (DTPA), EDTA, NADH, NAD⁺, recombinant E. coli formate dehydrogenase (FDH), rabbit L-lactate dehydrogenase, xanthine, bovine xanthine oxidase, and antibiotics were from Sigma Aldrich. Coomassie reagent was from Thermo Scientific. Formyl-Met-Ala-Ser was from Bachem.

Eben S.S, & Imlay J.A. (2023). Evidence that protein thiols are not primary targets of intracellular reactive oxygen species in growing Escherichia coli. Frontiers in Microbiology, 14, 1305973.

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Recombinant Metalloprotein Expression

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Brain heart infusion (BHI) broth, haemin, l-cysteine, l-cystine, fumaric acid, Fe(NH₄)₂(SO₄)₂, dithiothreitol, maltose, β-lactose, 2,2′-dipyridyl, citraconate, disodium l-malic acid, DL-trisodium isocitrate, xanthine and xanthine oxidase from bovine milk, catalase, E. coli iron-containing SOD, IPTG, imidazole, were bought from Sigma, d-glucose was obtained from Fisher Scientific, His Gravitrap, from GE Healthcare. Antibiotics (ampicillin and chloramphenicol) were all Sigma products.

Lu Z, & Imlay J.A. (2019). A conserved motif liganding the [4Fe–4S] cluster in [4Fe–4S] fumarases prevents irreversible inactivation of the enzyme during hydrogen peroxide stress. Redox Biology, 26, 101296.

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