Ntac sd females

All experimental procedures were approved by the University of Missouri’s Institutional Animal Care and Use Committee and were performed according to the guidelines set forth in the Guide for the Use and Care of Laboratory Animals. For production of rat embryos, NTac:SD females (4 weeks of age) and NTac:SD males (10 weeks of age) were purchased from Taconic Biosciences. For production of mouse embryos, C57BL/6J females (4 weeks of age) and C57BL/6J males (10 weeks of age) were purchased from the Jackson Laboratory. For rat embryo transfers, NTac:SD females (8 weeks of age) were purchased from Taconic Biosciences. For mouse embryo transfers, CD-1 females (8 weeks of age) were purchased from Charles River Laboratories. All animals were housed in ventilated cages (Thoren) and kept on a 12:12 light cycle. Food and water were available ad libitum.