As previously described, immunoblotting analyses were performed using the Odyssey Infrared Image System (LICOR Biosciences, Lincoln, NE, USA). Primary antibodies included anti‐TUBULIN (CST, #2146), anti‐HES1 (Santa Cruz, #166 410), anti‐HEY1 (Proteintech, #19929‐1‐AP), and anti‐HIF1A (ThermoFisher, PA1‐16601) antibodies. The secondary antibodies, IRDye 800CW goat anti‐rabbit (# 926 32211) and IRDye 680RD goat anti‐mouse (# 926 68070), were purchased from LI‐COR Biosciences (Lincoln, NE, USA). Quantification was normalized according to the expression of β‐TUBULIN.
Anti hey1
Manufactured by Proteintech
Sourced in United States
Anti‐HEY1 is a laboratory antibody product designed to detect the HEY1 protein. HEY1 is a transcriptional repressor that plays a role in various cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and study the HEY1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Irdye 680rd goat anti mouse, by LI COR (1 mentions)
Odyssey infrared image system, by LI COR (1 mentions)
Anti hif1a, by Thermo Fisher Scientific (1 mentions)
Irdye 800cw goat anti rabbit, by LI COR (1 mentions)
Anti hes1, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Trans blot turbo transfer system, by Bio-Rad (1 mentions)
Anti col1a1, by Proteintech (1 mentions)
Anti notch1, by Abcam (1 mentions)
Fast blocking buffer, by Ipsen (1 mentions)
Anti alp, by Abcam (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Anti runx2, by Proteintech (1 mentions)
Anti bmp2, by Proteintech (1 mentions)
2 protocols using anti hey1
1
Quantitative Immunoblotting Analyses
2
Protein Expression Analysis Protocol
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Equal amount of proteins were loaded onto 4-20% SDS-PAGE gels (Beyotime, Shanghai, China) and subjected to electrophoresis. PVDF membranes (Bio-Rad) were activated in methanol, and electrophoretic transfer was performed with the Trans-Blot Turbo Transfer system (Bio-Rad). The membranes were then immersed in fast-blocking buffer (EpiZyme, Shanghai, China) for 10 min, and probed with the indicated primary antibodies overnight in cold room. After being washed with TBST buffer, the membranes were incubated with secondary antibodies for 1 h at room temperature. The primary antibodies were listed as follows: anti-KLF9 (Abcam, Cambridge, UK), anti-GAPDH (Proteintech, Chicago, IL, USA), anti-ALP (Abcam), anti-Osx (Thermo Fisher Scientific, Waltham, MA, USA), anti-Dlx2 (Thermo Fisher Scientific), anti-BMP2 (Proteintech), anti-Runx2 (Proteintech), anti-COL1A1 (Proteintech), anti-Notch1 (Abcam), anti-HES1 (Thermo Fisher Scientific), and anti-HEY1 (Proteintech).
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