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Anti wdr5 antibody

Manufactured by Abcam
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The Anti-WDR5 antibody is a research-use only reagent designed to detect the WDR5 protein. WDR5 is a member of the WD repeat protein family and is involved in histone H3 methylation. This antibody can be used in various applications such as Western Blotting to identify and quantify the WDR5 protein.

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Lab products found in correlation

Ez magna rip kit, by Merck Group (1 mentions) Anti kat3b p300 antibody, by Abcam (1 mentions) Anti dot1l antibody, by Thermo Fisher Scientific (1 mentions) Anti tet1 antibody, by Abcam (1 mentions)

2 protocols using anti wdr5 antibody

1

Co-immunoprecipitation of WDR5, H3K4me3, and ALKBH4

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Coimmunoprecipitation was performed as described previously (Uyama et al., 2006 (link)). Briefly, HCT116 cells were harvested after indicated treatment. The whole-cell lysates were incubated with 2 μg of antibody or normal rabbit IgG at 4°C overnight, and two additional hours with 20 μl of 50% protein A agarose. Both input and IP samples were analyzed by western blot using various antibodies at the indicated dilutions: anti-WDR5 antibody (1:1000; Abcam), anti-H3K4me3 antibody (1:1000; CST) and anti- ALKBH4 (1:1000; Sigma).
Shen C., Yan T., Tong T., Shi D., Ren L., Zhang Y., Zhang X., Cao Y., Yan Y., Ma Y., Zhu X., Tian X., Fang J.Y., Chen H., Ji L., Hong J, & Xuan B. (2020). ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5. Frontiers in Cell and Developmental Biology, 8, 293.
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2

RNA-Protein Interaction Profiling

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RIP assays were performed using the EZ-Magna RIP Kit (Millipore). 1×10 7 cells were lysed with RIP lysis buffer. Cell extracts were coimmunoprecipitated with Anti-TET1 antibody (Abcam, ab191698), Anti-KAT3B/p300 antibody (Abcam, ab10485), Anti-WDR5 antibody (Abcam, ab56919), and Anti-DOT1L Antibody (Thermo, A300-953A).
The recovered RNA was subjected to RT-qPCR analysis and U1 was used as a nonspecific control target.
Zhou T., Zhu X., Ye Z., Wang Y., Yao C., Xu N., Zhou M., Ma J., Qin Y., Shen Y., Tang Y., Yin Z., Xu H., Zhang Y., Zang X., Ding H., Yang W., Guo Y., Harley J.B., Namjou B., Kaufman K.M., Kottyan L.C., Weirauch M.T., Hou G., & Shen N. (2021). CRISPRa screen on a genetic risk locus shared by multiple autoimmune diseases identifies a dysfunctional enhancer that affects IRF8 expression through cooperative lncRNA and DNA methylation machinery.
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