All proteins were resolved in 7.5% SDS-PAGE gels, except Sgs1 (6% SDS-PAGE gel), Rmi1 (10% SDS-PAGE gel), and histone H3 (15% SDS-PAGE gel). Proteins were transferred to polyvinylidene fluoride (PVDF) membranes using the TE70X semidry blotter system (Hoefer). The antibodies used were anti-HA (Roche, 3F10), anti-H3 (Abcam, ab1791), anti-myc (Roche, 9E10), anti-PGK1 (Thermo Scientific, 459250), anti-RFA (Agrisera, AS07 214), and anti-SMT3 (Abcam, ab14405). Blots were incubated with the ECL Prime Western blotting detection reagent (GE Healthcare) followed by exposure to high-performance chemiluminescence films (Amersham Hyperfilm ECL, GE Healthcare) to detect the signal.
Ab1791
Manufactured by Roche
Ab1791 is a laboratory equipment product manufactured by Roche. It is designed to perform specialized analytical tasks within a laboratory setting. The core function of Ab1791 is to facilitate precise and accurate measurements or analyses required for various research or diagnostic applications. The specific details of its intended use and capabilities are not provided in this factual, unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Ab14405, by Abcam (1 mentions)
Amersham hyperfilm ecl, by GE Healthcare (1 mentions)
Te70x semidry blotter system, by Hoefer (1 mentions)
As07 214, by Abcam (1 mentions)
Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by GE Healthcare (1 mentions)
As09532a, by Abcam (1 mentions)
2 protocols using ab1791
1
Western Blot Analysis of Protein Complexes
2
Immunoblotting of Cellular Proteins
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Protein samples were separated by SDS-PAGE gel and transferred to PVDF membranes.
Antibodies against FCA 39 , SE (Agrisera, AS09532A), HYL1 40 (link) , ELF3 41 (link) , H3 (Abcam, ab1791), GFP (Roche, 11814460001), Tubulin (Sigma, T5168) were used as primary antibodies. After the primary antibody incubation, horseradish peroxidase (HRP)conjugated secondary antibodies (GE Healthcare) were used for protein detection by chemiluminescence (Thermo Scientific, 34095).
Antibodies against FCA 39 , SE (Agrisera, AS09532A), HYL1 40 (link) , ELF3 41 (link) , H3 (Abcam, ab1791), GFP (Roche, 11814460001), Tubulin (Sigma, T5168) were used as primary antibodies. After the primary antibody incubation, horseradish peroxidase (HRP)conjugated secondary antibodies (GE Healthcare) were used for protein detection by chemiluminescence (Thermo Scientific, 34095).
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