Total RNA was extracted with RNAzol ® RT reagent (Sigma-Aldrich ® , R4533) following the manufacturer's instructions and eluted in RNAse-free water. Concentration was determined with the Nanophotometer TM P-Class (Implem GmbH, P330). Integrity was verified by gel red staining of 28S and 18S ribosomal RNA bands on a 1% agarose gel. cDNA was retrotranscribed from 3 µg RNA using the Tetro Reverse Transcriptase cDNA synthesis kit (Bioline, BIO-65050). Nucleic acids were kept at -20°C until use.
Tetro reverse transcriptase cdna synthesis kit
Manufactured by Meridian Bioscience
The Tetro Reverse Transcriptase cDNA synthesis kit is a laboratory product used for the conversion of RNA into complementary DNA (cDNA) molecules. The kit contains the necessary components, including the Tetro Reverse Transcriptase enzyme, to facilitate this process.
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Lab products found in correlation
2 protocols using tetro reverse transcriptase cdna synthesis kit
1
Total RNA Extraction and cDNA Synthesis
2
RNA Extraction and cDNA Synthesis
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Tissues were stored in RNAlater until RNA extraction was performed with RNAzol® RT reagent (SIGMA-ALDRICH ® , R4533) following the manufacturer's instructions. Elution occurred in RNAse-free water. Concentration was determined through the Nanophotometer TM P-Class (Implem GmbH, Munich, Germany), while integrity was verified by gel red staining of 28S and 18S ribosomal RNA bands on a 1% agarose gel. Tetro Reverse Transcriptase cDNA synthesis kit (Bioline, BIO-65050) was used to synthesize cDNA from 3 µg of RNA. Nucleic acids were then kept at -20 °C until use.
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