Anti βactin antibody

Manufactured by Keygen Biotech

Sourced in China

Anti-βactin antibody is a laboratory reagent used to detect and quantify the presence of the beta-actin protein, a ubiquitous cytoskeletal protein found in all eukaryotic cells. It is commonly used as a loading control in Western blotting and other protein analysis techniques.

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Lab products found in correlation

Caov3, by American Type Culture Collection (1 mentions) P p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) P70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) Skov3, by American Type Culture Collection (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Elisa plate reader, by Bio-Rad (1 mentions) Ecl detection kit, by Beyotime (1 mentions) Mtt detection kit, by Beyotime (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Bca kit, by Beyotime (1 mentions)

2 protocols using anti βactin antibody

Resistin Regulates Ovarian Cancer Progression

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Two human ovarian epithelial carcinoma cell lines (CAOV3 and SKOV3) were purchased from ATCC (Manassas, VA, USA). McCoy5A medium was purchased from HyClone (Logan, UT, USA). Recombinant resistin, mTOR antibody, rapamycin, adult fat mesenchymal stem cells, and adult fat mesenchymal stem cells complete medium were purchased from Phoenix Pharmaceuticals (Phoenix Pharmaceuticals, Mannheim, Germany). Anti-βactin antibody was obtained from Keygen Biotech (1:1000, Nanjing, China). Phospho (p)- mTOR, mTOR, p- p70S6 kinase (Thr389), and p70S6 kinase (Thr389) were purchased from Cell Signaling Technology (1:1000, Danvers, MA, USA). Cell Counting Kit-8 (CCK-8) was supplied by Dojindo (Kumamoto, Japan), and the ELISA plate reader was obtained from Bio-Rad (Hercules, CA, USA).

Pang L, & Chang X. (2021). Resistin Expression in Epithelial Ovarian Cancer promotes the Proliferation and Migration of Ovarian Cancer Cells to Worsen Prognosis. Journal of Cancer, 12(22), 6796-6804.

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Immunofluorescence Labeling Optimization Protocol

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Lipofectamine 2000 transfection reagent (Cat. No. 11668027), anti-GFP antibody (Cat. No. A11122), FITC-conjugated goat anti-rabbit (Cat. No. A16097), Alexa flour 488-conjugated goat anti-rabbit IgG (Cat. No.A11008), and Alexa flour 568-conjugted goat anti-mouse IgG (Cat. No.A11004) antibodies were purchased from Invitrogen. Antibody against MIM (Cat. No. PA5-17047), Cell Dissociation Buffer (Cat. No. 13151014), protein A/G agarose beads (Cat. No. 20423) and G418 sulfate (Cat. No. 10131035) were purchased from Thermo Scientific. Anti-CD107a (LAMP-1) antibody (Cat. No. sc20011) was purchased from Santa Cruz. Anti-EEA1 (Cat. No. ab70521) and anti-CD63 (Cat. No. ab213090) antibodies were from Abcam. Protease Inhibitor Cocktail (Cat. No. P8340), monoclonal antibodies against clathrin light chain (Cat. No. C1985), clathrin heavy chain (Cat. No. C1860), Rab7 (Cat. No.R8779) and anti-Rab5 (Cat. No. R7904) were purchased from Sigma-Aldrich. Phosphate-Buffered Saline (PBS) (Cat. No. 21-040-CV) was purchased from Corning. Anti-β-actin antibody was from Keygen Biotech. MTT detection kit, RIPA lysis buffer, BCA kit and ECL detection kit were purchased from Beyotime Biotech.

Zhao P., Chen B., Li L., Wu H., Li Y., Shaneen B., Zhan X, & Gu N. (2018). Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7. Biochimica et biophysica acta. General subjects, 1863(2), 502-510.

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