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2 protocols using pheophytin a

1

Antioxidant Capacity Assessment Protocol

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Ammoniac (NH3), ammonium acetate, boric acid, ferulic acid, hydrochloric acid (HCl), kaempferol-3-O-glucoside, methanol, natrium hydroxide (NaOH), quercetin-3-O-glucoside, sinapic acid, and tert-butyl methyl ether were obtained from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Acetic acid, 2-propanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), methylene chloride, potassium persulfate, and Trolox® (6-hydroxyl-2,5,7,8-tetramethylchroman-2carbonic acid) were purchased from Merck KGaA (Darmstadt, Germany). 2,2′-Azinobis-(3-ethylbenzthiazoline-6sulfonic acid) (ABTS) was purchased from Roche Diagnostic GmbH (Mannheim, Germany) and sulfuric acid from Grüssing GmbH (Filsum, Germany). Acetonitrile was obtained from J.T. Baker (Fisher Scientific GmbH, Grießheim, Germany) and tetrahydrofurane was from VWR International GmbH (Darmstadt, Germany). The standards of β-carotene, chlorophyll a, chlorophyll b, lutein, and zeaxanthin were purchased from CaroteNature® GmbH (Münsingen, Switzerland) and pheophytin a from LGC Standards GmbH (Wesel, Germany).
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2

Comprehensive Phytochemical Analysis Protocol

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Ammonium acetate, genistein, kaempferol-3-O-glucoside, methanol, quercetin-3-O-glucoside, and tert-butyl methyl ether were purchased from Roth (Carl Roth GmbH, Karlsruhe, Germany). Acetic acid, 2-propanol, and dichloromethane were obtained from Merck (Merck KGaA, Darmstadt, Germany); acetonitrile from J. T. Baker (Fisher Scientific GmbH, Grießheim, Germany) and tetrahydrofurane (THF) from VWR (VWR International GmbH, Darmstadt, Germany). Standards of β-carotene, lutein, and zeaxanthin were from CaroNature (CaroteNature GmbH, Münsingen, Switzerland). Chlorophyll a and chlorophyll b were purchased from Sigma-Aldrich (Sigma Aldrich Chemie GmbH Taufkirchen, Germany). Chrysoeriol was obtained from Phytolab (Phytolab GmbH & Co. KG, Vestenbergsgreuth, Germany) and pheophytin a from LGC Standards (LGC Standards GmbH Wesel, Germany). All chemicals were of HPLC-MS-grade quality.
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