For determination of cell cycle distribution and percentage of cell apoptosis, a cell cycle assay kit and an AnnexinV/PI apoptosis kit (US Everbright Inc) were respectively used following to their manufacturer’s protocols. Two hours before cell apoptosis analysis, GC cells were treated with an apoptosis inducer CCCP at a concentration of 150 mM.
Cell cycle assay kit
Manufactured by US Everbright
The Cell Cycle Assay Kit is a laboratory tool designed to analyze the progression of cells through the different phases of the cell cycle. It provides a quantitative assessment of the proportion of cells in the G0/G1, S, and G2/M phases.
Automatically generated - may contain errors
Lab products found in correlation
Victor nivo microplate reader, by PerkinElmer (1 mentions)
Bk011p, by Cytoskeleton (1 mentions)
Anti paxillin, by Santa Cruz Biotechnology (1 mentions)
Mouse anti c src, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Nci h1688, by American Type Culture Collection (1 mentions)
Nci h446, by American Type Culture Collection (1 mentions)
2 protocols using cell cycle assay kit
1
Cell Cycle and Apoptosis Analysis
2
In Vitro and In Vivo Evaluation of Novel Anticancer Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NCI-H1688, SK-OV-3, MDA-MB-231,
HT-29, HepG2, and NCI-H446 cell lines were purchased from the American
Type Culture Collection (Rockville, MD, USA). McCoy’s 5A, DMEM,
RPMI-1640 medium, and fetal bovine serum (FBS) were purchased from
Gibco (Gaithersburg, MD, USA); the absorbance and fluorescence were
measured using a Victor Nivo Microplate Reader (Perkin Elmer, German);
a Cell Cycle Assay Kit was purchased from US Everbright Inc. (Suzhou,
China); a tubulin polymerization assay kit (BK011P) was purchased
from Cytoskeleton Inc. (Denver, CO, USA); Western blot analyses were
performed with primary antibodies against the following antigens:
mouse anti-phospho-c-Src (1:100; Santa Cruz Cat# sc-81521), mouse
anti-c-Src (1:200, Santa Cruz Cat# sc-8056), anti-paxillin (pY118)
antibody (1:500; Cohesion Biosciences Cat# CPA3307), anti-paxillin
(1:500; Santa Cruz Cat# sc-365379), rabbit anti-GAPDH (1:1000; Cell
Signaling Technology Cat# 2118), anti-rabbit IgG, HRP-linked antibody
(1:4000; Cell Signaling Technology Cat# 7074), anti-mouse IgGκ,
and HRP-linked antibody (1:4000; Santa Cruz Cat# sc-516102). Five-week-old
female non-obese diabetic (NOD)/severe-combined immunodeficient mouse
(SCID) and male Sprague–Dawley rats were obtained from Weitonglihua
(Beijing, China).
HT-29, HepG2, and NCI-H446 cell lines were purchased from the American
Type Culture Collection (Rockville, MD, USA). McCoy’s 5A, DMEM,
RPMI-1640 medium, and fetal bovine serum (FBS) were purchased from
Gibco (Gaithersburg, MD, USA); the absorbance and fluorescence were
measured using a Victor Nivo Microplate Reader (Perkin Elmer, German);
a Cell Cycle Assay Kit was purchased from US Everbright Inc. (Suzhou,
China); a tubulin polymerization assay kit (BK011P) was purchased
from Cytoskeleton Inc. (Denver, CO, USA); Western blot analyses were
performed with primary antibodies against the following antigens:
mouse anti-phospho-c-Src (1:100; Santa Cruz Cat# sc-81521), mouse
anti-c-Src (1:200, Santa Cruz Cat# sc-8056), anti-paxillin (pY118)
antibody (1:500; Cohesion Biosciences Cat# CPA3307), anti-paxillin
(1:500; Santa Cruz Cat# sc-365379), rabbit anti-GAPDH (1:1000; Cell
Signaling Technology Cat# 2118), anti-rabbit IgG, HRP-linked antibody
(1:4000; Cell Signaling Technology Cat# 7074), anti-mouse IgGκ,
and HRP-linked antibody (1:4000; Santa Cruz Cat# sc-516102). Five-week-old
female non-obese diabetic (NOD)/severe-combined immunodeficient mouse
(SCID) and male Sprague–Dawley rats were obtained from Weitonglihua
(Beijing, China).
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