HP-HILIC (Waters BEH amide, 2.1 mm × 100 mm × 2.5 µm, equipped with HP1100 quad pump HPLC) coupled with tandem mass spectrometers (Thermo Scientific LTQ XL for structural identification and pathway analysis of 3-HIB, or Thermo Scientific Q Exactive orbitrap for high-sensitivity quantitation of in vitro and in vivo samples) was used. Ammonium formate buffer (2 mM adjusted at pH 9.0) in water/acetonitrile mix was used as eluent, initially at water:acetonitrile = 10:90 for 4 min, ramped to 40:60 over 3 min and held for 5 min for washing and reconditioned for 12 min. Selective ion monitoring (SIM)/parallel reaction monitoring (PRM) at 103.1 (hydroxybutyrate (HB) isomers), 106.1 (d3-2-HB standard) and 109.1 (d6-4-HB standard) were carried out for MS2 spectra acquisition. Quantification of each HB species was carried out with specific Q1→Q3 transition fingerprint (2-HB: 103→57, 3-HB:103→59, 3-HIB:103→73, 4-HB:103→85). 13C-2-Valine was purchased from Sigma (#604917). 13C2-1,2-Leucine was purchased from Cambridge Isotope Laboratories (#CLM-3524). d3-2HB was purchased from CDN Isotopes (#D-7002) and d6-4HB was purchased from Cerilliant (#G-006).
13c 2 valine
Manufactured by Merck Group
13C-2-Valine is a stable isotope labeled amino acid. It is used as a research tool in various applications, including metabolic studies and tracer experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 13c 2 valine
1
Quantitative Analysis of Hydroxybutyrates
2
Quantitative Analysis of Hydroxybutyrates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HP-HILIC (Waters BEH amide, 2.1 mm × 100 mm × 2.5 µm, equipped with HP1100 quad pump HPLC) coupled with tandem mass spectrometers (Thermo Scientific LTQ XL for structural identification and pathway analysis of 3-HIB, or Thermo Scientific Q Exactive orbitrap for high-sensitivity quantitation of in vitro and in vivo samples) was used. Ammonium formate buffer (2 mM adjusted at pH 9.0) in water/acetonitrile mix was used as eluent, initially at water:acetonitrile = 10:90 for 4 min, ramped to 40:60 over 3 min and held for 5 min for washing and reconditioned for 12 min. Selective ion monitoring (SIM)/parallel reaction monitoring (PRM) at 103.1 (hydroxybutyrate (HB) isomers), 106.1 (d3-2-HB standard) and 109.1 (d6-4-HB standard) were carried out for MS2 spectra acquisition. Quantification of each HB species was carried out with specific Q1→Q3 transition fingerprint (2-HB: 103→57, 3-HB:103→59, 3-HIB:103→73, 4-HB:103→85). 13C-2-Valine was purchased from Sigma (#604917). 13C2-1,2-Leucine was purchased from Cambridge Isotope Laboratories (#CLM-3524). d3-2HB was purchased from CDN Isotopes (#D-7002) and d6-4HB was purchased from Cerilliant (#G-006).
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