Calf intestine alkaline phosphatase grade I was bought from Roche Diagnostic (Indianapolis, IN). Phosphodiesterase I from Crotalus adamanteus venom, deoxyribonuclease I type II from bovine pancreas, dA, dA 3′-monophosphate (dA-3′-P), dA 5′-monophosphate (dA-5′-P), dG, dG 3′-monophosphate, micrococcal nuclease (MN), RNase A, RNase T1, protease, human placental DNA, calf thymus DNA, glycidaldehyde diethyl acetal, Amberlyst 15 ion exchange resin, dietary vitamin E (α-tocopherol), α-lipoic acid were obtained from Sigma-Aldrich Co. (St. Louis, MO). Polyphenon E was a generous gift from Dr. Hara (Mitsui Norin, Japan). [15N5]-dA was from Spectra Stable Isotopes (Columbia, MD). Spleen phosphodiesterase (SPD) was obtained from Boehringer Mannheim (Indianapolis, IN), T4 polynucleotide kinase (T4 PNK) was from U.S. Biochemicals (Cleveland, OH). [γ-32P]-ATP (specific activity 3000 Ci/mmole) was from Amersham (Arlington Heights, IL) and mung bean nuclease (MBN) was from Thermo Fisher Scientific (Fair Lawn, NJ). All other chemicals, solvents and reagents were from Sigma-Aldrich Co. (St. Louis, MO) or Thermo Fisher Scientific (Fair Lawn, NJ).
Amberlyst 15 ion exchange resin
Manufactured by Merck Group
Sourced in United States
Amberlyst 15 is a strongly acidic cation exchange resin produced by Merck. It is a macroporous polymer with a high degree of cross-linking and sulfonic acid functional groups. Amberlyst 15 is designed for use in various chemical processes and reactions that require an acidic catalyst or ion exchange medium.
Automatically generated - may contain errors
Lab products found in correlation
γ 32p atp, by Cytiva (1 mentions)
Calf thymus dna, by Merck Group (1 mentions)
Rnase a, by Merck Group (1 mentions)
Phosphodiesterase 1, by Merck Group (1 mentions)
α lipoic acid, by Merck Group (1 mentions)
Human placental dna, by Merck Group (1 mentions)
Micrococcal nuclease, by Merck Group (1 mentions)
Rnase t1, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
D fructose, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Ethanol absolute anhydrous, by Carlo Erba (1 mentions)
2 20 azino bis, by Merck Group (1 mentions)
3 protocols using amberlyst 15 ion exchange resin
1
Nuclease and Phosphatase Assays
2
Quantitative Carbohydrate Analysis
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Methanol (HPLC grade) and ethanol absolute anhydrous were purchased from Carlo Erba (Milan, Italy); sodium carbonate, water (LC-MS, Ultra Chromasolv, Honeywell, Seelze, Germany), and acetonitrile (LC-MS Chromasolv > 99.9%) from Honeywell (Seelze, Germany). Formic acid (99% LC-MS grade) was purchased from VWR Chemicals (Radnor, PA, USA). Amberlyst 15 ion-exchange resin, Folin–Ciocalteu reagent, ABTS (2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-picrylhydrazyl) were purchased from Sigma-Aldrich (St. Louis, USA). Standard of D-(+)-saccharose molecular biology grade was purchased from AppliChem GmbH (Darmstadt, Germany); standards of D-(+)-raffinose and stachyose tetrahydrate were purchased from Supelco analytical (Darmstadt, Germany); standard of L-(−)-xylose and D-(−)-fructose were purchased from Sigma (St. Louis, MO, USA).
3
Synthesis and Deblocking of P6C/AASA
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The synthesis of P6C/AASA was performed as described previously (3 (link)). Allysine ethylene acetal (10 mg; Sigma-Aldrich, 714208) was deblocked by mixing with Amberlyst 15 ion exchange resin (40 mg; Sigma-Aldrich, 216380) in 1 ml of water. The Amberlyst 15 was then used to prepare a solid-phase column and was washed twice with 1 ml of water. P6C/AASA was eluted from the resin with 1.5 ml of 25% ammonia solution (RHAWN, R051454) and was dried under nitrogen at room temperature before resuspending in 1 ml of water.
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