Zinc (II) protoporphyrin IX (ZnPP), protoporphyrin IX (PPIX), adenosine 5′-triphosphate disodium salt hydrate (ATP), and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) were purchased from Merck (Darmstadt, Germany). HPLC gradient-grade acetonitrile (ACN) and trifluoroacetic acid (TFA) were obtained from Merck (Darmstadt, Germany). Porcine hemin chloride (heme) was obtained from PanReac AppliChem (Barcelona, Spain). Food-grade ascorbic acid (E-300) was purchased from Collelldevall SL (Banyoles, Spain). All other reagents used in this study were of ACS grade. Distilled water was obtained using a Milli-Q system (Merck Millipore, Darmstadt, Germany).
Hplc gradient grade acetonitrile acn
Manufactured by Merck Group
Sourced in Germany
HPLC gradient-grade acetonitrile (ACN) is a high-purity solvent used in high-performance liquid chromatography (HPLC) analysis. It serves as a mobile phase component to facilitate the separation and detection of various analytes during HPLC experiments.
Automatically generated - may contain errors
Lab products found in correlation
Methanol meoh, by Merck Group (2 mentions)
Milli q system, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid disodium salt dihydrate, by Merck Group (1 mentions)
Protoporphyrin 9, by Merck Group (1 mentions)
Adenosine 5 triphosphate disodium salt hydrate, by Merck Group (1 mentions)
Glycidyl methacrylate, by Merck Group (1 mentions)
Trisodium citrate, by Merck Group (1 mentions)
Chlorothalonil, by Merck Group (1 mentions)
Cyclohexanol, by Thermo Fisher Scientific (1 mentions)
1 dodecanol, by Thermo Fisher Scientific (1 mentions)
Azoxystrobin, by Merck Group (1 mentions)
Ethylene dimethacrylate, by Merck Group (1 mentions)
3 protocols using hplc gradient grade acetonitrile acn
1
Heme Quantification Using HPLC-UV
2
Adsorption of Compounds on Silica Gel
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Investigated compounds (Table 1 ) were obtained from Sigma (St. Louis, MO, USA) except for 1-ethyl-3-methylimidazolium hexafluorophosphate (EMIM PF6), which was from Fluka (Sigma-Aldrich Group, Lausanne, Switzerland). HPLC gradient-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Merck (Darmstadt, Germany). Silica gel (LiChrospher Si 1000, mean particle size 10 µm) used as adsorbent was obtained from Merck. Prior to the adsorption process, the adsorbent was washed with distilled water to eliminate impurities, dried at 120 °C for 2 h. HPLC water was obtained from a Barnstead Deionising System (Dubuque, IA, USA). All mobile phases were buffered by the phosphate buffer (pH: 2.9–3.0). Its concentration was 30 or 50 mmol·L−1 in the whole mobile phase. The eluents were prepared by mixing the buffer solution, organic solvent and appropriate amounts of sodium hexafluorophosphate, sodium tetrafluoroborate.
3
Pesticide Analysis Protocol Development
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Chlorothalonil (CLO) (99.3%), azoxystrobin (AZO) (99.4%), glycidyl methacrylate (GMA), ethylene dimethacrylate (EDMA) and trisodium citrate were purchased from Sigma-Aldrich (Steinheim, Germany, http://www.sigmaaldrich.com). Azobisisobutyronitrile (AIBN) was from Fluka (Buchs, Switzerland, http://www.sigmaaldrich.com). HPLC gradient grade acetonitrile (ACN) and methanol (MeOH) were from Merck (Darmstadt, Germany, http://www.merck.com). AuNP suspension (particle size, 20 nm, stabilized with sodium citrate, 6.5410 11 particles per mL, 1.0910 -9 M), cyclohexanol and 1dodecanol were from Alfa Aesar (Landcashire, United Kingdom, http://www.alfa.com). Ethanol and ammonia were from Scharlab (Barcelona, Spain, http://www.scharlab.com). A ultra-pure water system Puranity TU 6 from VWR (Germany, http://www.vwr.com) was used for water purification.
Stock solutions of CLT and AZO (500 mg L -1 ) were prepared by dissolving appropriate amounts of each pesticide in ACN, and working standard solutions were obtained by dilution of the stock solutions with deionized water.
Stock solutions of CLT and AZO (500 mg L -1 ) were prepared by dissolving appropriate amounts of each pesticide in ACN, and working standard solutions were obtained by dilution of the stock solutions with deionized water.
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