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Anti human iga α chain specific

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Anti-human IgA (α-chain specific) is a laboratory reagent used for the detection and quantification of human IgA antibodies in various biological samples. It is a highly specific antibody that binds to the α-chain of the human IgA immunoglobulin.

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Lab products found in correlation

Tetra cell, by Bio-Rad (2 mentions) Anti rabbit igg f ab 2 specific, by Jackson ImmunoResearch (2 mentions) Pierce protein free tbs blocking buffer, by Thermo Fisher Scientific (2 mentions) Anti rabbit igg whole molecule, by Merck Group (2 mentions) Semi dry transfer apparatus, by Bio-Rad (2 mentions)

2 protocols using anti human iga α chain specific

1

Immunoblotting of Mouse Serum and Lavage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse samples (10μl serum diluted 1:1000, or 20μl undiluted nasal lavage) were resolved on a 10% Mini-PROTEAN®TGX™ gel using a Tetra cell (Bio-Rad, Hercules, CA). Reduced samples were prepared with Laemmli sample buffer containing β-mercaptoethanol (βME) and boiled for 5 mins. Non-reduced samples were prepared without βME and boiling. Gels were transferred onto PVDF membranes by semi-dry transfer apparatus (Bio-Rad). Membranes were blocked in Pierce Protein-Free (TBS) Blocking Buffer (Thermo Fisher Scientific). Whole rabbit IgG was detected in lavages using anti-rabbit IgG (whole molecule) (Sigma, St. Louis, MO). Rabbit IgG fragments were detected using anti-rabbit IgG (F(ab’)2 specific) (Jackson ImmunoResearch Laboratories, West Grove, PA). hmAbs were detected using anti-human IgA (α-chain specific) (Sigma).
Roche A.M., Richard A.L., Rahkola J.T., Janoff E.N, & Weiser J.N. (2014). Antibody blocks acquisition of bacterial colonization through agglutination. Mucosal immunology, 8(1), 176-185.
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2

Immunoblotting of Mouse Serum and Lavage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse samples (10μl serum diluted 1:1000, or 20μl undiluted nasal lavage) were resolved on a 10% Mini-PROTEAN®TGX™ gel using a Tetra cell (Bio-Rad, Hercules, CA). Reduced samples were prepared with Laemmli sample buffer containing β-mercaptoethanol (βME) and boiled for 5 mins. Non-reduced samples were prepared without βME and boiling. Gels were transferred onto PVDF membranes by semi-dry transfer apparatus (Bio-Rad). Membranes were blocked in Pierce Protein-Free (TBS) Blocking Buffer (Thermo Fisher Scientific). Whole rabbit IgG was detected in lavages using anti-rabbit IgG (whole molecule) (Sigma, St. Louis, MO). Rabbit IgG fragments were detected using anti-rabbit IgG (F(ab’)2 specific) (Jackson ImmunoResearch Laboratories, West Grove, PA). hmAbs were detected using anti-human IgA (α-chain specific) (Sigma).
Roche A.M., Richard A.L., Rahkola J.T., Janoff E.N, & Weiser J.N. (2014). Antibody blocks acquisition of bacterial colonization through agglutination. Mucosal immunology, 8(1), 176-185.
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