Sifoxo3a

Manufactured by RiboBio

Sourced in China

SiFOXO3a is a laboratory equipment designed for scientific research. It functions as a tool for the analysis and study of specific cellular processes and pathways.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using sifoxo3a

Silencing HIF-1α and FOXO3a in HTR8/SVneo Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific siRNAs for HIF-1α (siHIF-1α; 5′-CAATCAAGAAGTTGCATTA-3′) and FOXO3a (siFOXO3a; 5′-GCACAGAGTTGGATGAAGT-3′) were purchased from Guangzhou RiboBio Co., Ltd. Then, 250 µl RNase-free water was used to the synthesize siRNAs until they reached a storage concentration of 20 µM. HTR8/SVneo cells were cultured in 6-well plates (1×10⁵/well). Cells with 60% confluence were replaced with fresh culture serum-free medium 2 h prior to transient transfection. Using a final concentration of 50 nM siHIF-1α, siFOXO3a and negative control (siNC), 5 µl siRNAs were transfected into cells using 5 µl Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). After 48 h of transfection, extraction of total RNA or protein of cells was assessed to confirm efficiency for subsequent experiments.

Zhang Z., Huang C., Wang P., Gao J., Liu X., Li Y., Yan S, & Shi Y. (2020). HIF-1α affects trophoblastic apoptosis involved in the onset of preeclampsia by regulating FOXO3a under hypoxic conditions. Molecular Medicine Reports, 21(6), 2484-2492.

+ Open protocol

+ Expand

Molecular Mechanisms of CUL4B-Mediated Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human SCC cell lines, NCI-H520 and SK-MES-1, and human SCLC cell lines, DMS114 and NCI-H2227, were obtained from the American Type Culture Collection (Manassas, VA, USA). Antibodies were purchased from commercial sources, including rabbit anti-CUL4B antibody (1:50; Novus Biologicals, Littleton, CO, USA), rabbit anti-CUL4A (1:1,000; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-FOXO3A (1:1,000; Cell Signaling Technology), rabbit anti-p-FOXO3A (1:1,000; Cell Signaling Technology), rabbit anti-cyclin E1 (1:1,000; Abcam, Cambridge, UK), rabbit anti-ERK (1:1,000; Abcam), rabbit anti-p-ERK (1:1,000; Abcam), rabbit anti-P21 (1:1,000; Proteintech, Rosemont, IL, USA), rabbit anti-CUL4B (1:1,000; Proteintech), rabbit anti-BCL-2 (1:1,000; Proteintech), rabbit anti-BAX (1:1,000; Proteintech), rabbit anti-caspase 3 (1:1,000; Proteintech), rabbit anti-UBC12 (1:1,000; Proteintech), and mouse anti-β-actin (1:1,000; Sigma-Aldrich, St. Louis, MO, USA). Si-RNAs were purchased from RiboBio (Guangzhou, China), including si-FOXO3A and si-UBC12. MG132, MLN4924, and PD98059 were purchased from Selleck Chemicals (Houston, TX, USA). Cycloheximide (CHX) was purchased from Absin (Shanghai, China).

Li T., Wu S., Jia L., Cao W., Yao Y., Zhao G, & Li H. (2020). CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma. Cancer Biology & Medicine, 17(2), 357-370.

+ Open protocol

+ Expand

Lung Cancer Cell Line Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The lung cancer cell lines PC9, A549, H1975, H1299 and H358 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone, Thermo Fisher, USA) containing 0.1 mM sodium pyruvate, 10% FBS, 2 mM l-glutamine, 100 IU/ml penicillin, and 100 μg/ml streptomycin at 37 °C in a chamber (5% CO₂ atmosphere). Cells (2 × 10⁶/per well) were seeded in 6-well plates and transfected with 4 μg of the pEGFP-N1 (control), pEGFP-FoxO3a or pEGFP-EPS8, 5 μl of si-FoxO3a or si-EPS8 (Ribobio, China) using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA) in antibiotic-free medium. After 48 h, the transient cells were collected for further experiments.

Wen Q., Jiao X., Kuang F., Hou B., Zhu Y., Guo W., Sun G., Ba Y., Yu D., Wang D., Zhang F., Qiao H.C., Wang S., Tang S, & Qiao H. (2019). FoxO3a inhibiting expression of EPS8 to prevent progression of NSCLC: A new negative loop of EGFR signaling. EBioMedicine, 40, 198-209.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!