High-resolution transmission electron microscopy (HR-TEM) and energy dispersive spectroscopy (EDS) analyses were performed using a JEM-2100F microscope set at an accelerating voltage of 200 kV. The particle size distribution and zeta potential were evaluated through nanoparticle tracking analysis (NTA) using the ZetaView instrument (Particle Metrix, Inning am Ammersee, Germany). The quantity of cerium that was incorporated into MSCExo-Ce was established via inductively coupled plasma mass spectrometry (ICP-MS) using an iCAP Qc system (Thermo Fisher Scientific, Waltham, MA, USA), and the cerium was subsequently normalized based on the vesicle count.
Icap qc system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICAP Qc system is an inductively coupled plasma (ICP) spectrometer designed for qualitative and quantitative analysis of a wide range of elements in various sample types. The system utilizes a high-temperature plasma to atomize and ionize the sample, and an optical detector to measure the characteristic emission spectra of the elements present. The ICAP Qc system is capable of analyzing multiple elements simultaneously with high sensitivity and precision.
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Lab products found in correlation
2 protocols using icap qc system
1
Characterizing Cerium-Enriched Extracellular Vesicles
2
Elemental Mapping of Cryogel Membranes
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The cryogels were washed with ultrapure water and freeze-dried. Inductively coupled plasma mass spectrometry (ICP-MS) analysis was performed on an iCAP Qc system (Thermo Fisher Scientific). The samples were dissolved in a mixture of 1 part of 38% HCl and 9 parts of 68% HNO3 (ultra-pure grade). HCl/HNO3 mixture alone was used as a blank solution. The samples were decomposed in hermetic PTFE vials in a microwave oven Mars 6 (CEM Corporation) at 210°C. The final solution was diluted by ultrapure water and HCl; overall acid concentration was maintained at 2%. Internal In standard was added to analyzed solutions at a final concentration of 5 ppb. The mass-spectrometer was pre-calibrated using multi-element standards in the range of concentration 1-100 ppb. The data were presented as mean ± SD (n=3).
Micro X-ray fluorescent (µ-XRF) analysis was performed on a M4 Tornado analyzer (Bruker) with a rhodium X-ray tube (30 W) and the point size of 25 µm. Dried cryogel membranes were attached to the sample stage, and µ-XRF spectra from the upper surface were recorded with a 4 µm step under vacuum. Based on the spectra, 2D maps of the samples were depicted where an individual color and its intensity corresponded to an element and its content. Back color designated lack of element(s) or hollow spaces.
Micro X-ray fluorescent (µ-XRF) analysis was performed on a M4 Tornado analyzer (Bruker) with a rhodium X-ray tube (30 W) and the point size of 25 µm. Dried cryogel membranes were attached to the sample stage, and µ-XRF spectra from the upper surface were recorded with a 4 µm step under vacuum. Based on the spectra, 2D maps of the samples were depicted where an individual color and its intensity corresponded to an element and its content. Back color designated lack of element(s) or hollow spaces.
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