Mouse caspase 1 clone 4b4

Ultra-pure flagellin (Psuedomonas aeruginosa), Pam3CSK4, R837 (Imiquimod), ultra-pure LPS (E. coli O111:B4), Poly(dA:dT), Nigericin were purchased from Invivogen, Mega FasL from AdipoGen, IFN-α and IFN-β from PBL Assay Science, TNFα from Genentech, ATP and bovine cytochrome-c from Sigma, YOYO-1 dye from Thermofisher Scientific, Nuclear-ID DNA stain from Enzo Life Sciences. Antibodies used include: mouse caspase-1 (clone 4B4, Genentech), Gsdmd (17G2G9, Genentech), caspase-3 (Cell Signaling Technology), cleaved active caspase-3 (5A1E, Cell Signaling Technology), caspase-8 (1G12, Enzo), cleaved active caspase-8 (D5B2, Cell Signaling Technology), IL-1β (GTX74034, GeneTex) and actin (AC15, NOVUS) and DFNA5 rabbit polyclonal antibody was raised against ESDFVKYESKCENHKSGAIG peptide (mouse DFNA5 74–93 amino acid) and FLAG M2 antibody (Sigma).

Immunoblot was performed as described previously (Zhang et al., 2021b (link)). Antibodies used included: mouse caspase-1 (clone 4B4) [a kind gift from V. M. Dixit (Genentech, USA)], mouse GSDMD (ab209845, Abcam, UK), pro-caspase-8 (4790, Cell Signaling Technology, USA), mouse cleaved-caspase-8 (9429, Cell Signaling Technology), mouse RIP3 (homemade), mouse MLKL (homemade), human caspase-8 (ALX-804-242-C100, Enzo Life Sciences, USA), caspase-3 (9662, Cell Signaling Technology), human RIP3 (13526, Cell Signaling Technology), p38α (9228, Cell Signaling Technology), p-p38 (9211, Cell Signaling Technology), ERK (9107, Cell Signaling Technology), p-ERK (4377, Cell Signaling Technology), JNK (9252, Cell Signaling Technology), p-JNK (9251, Cell Signaling Technology), mouse p38α (homemade), Tubulin (M20005M, Abmart, China), Actin (homemade), and GAPDH (AC002, ABclonal, China).