The supernatant of the tissue explant culture was kept frozen at −80 °C until used for ELISA. ELISA detection kits for murine IL-6 and IL-23 (BMS603HS and BMS6017, eBiosciences) were used according to the manufacturer’s instructions. Absorbance was determined using a POLARstar Optima plate reader (BMG Labtech).
Bms603hs
Manufactured by Thermo Fisher Scientific
The BMS603HS is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a high-speed benchtop centrifuge designed for a variety of applications in research and clinical settings. The BMS603HS provides consistent and reliable performance with a maximum speed of 15,000 rpm and a maximum relative centrifugal force of 21,382 x g.
Automatically generated - may contain errors
6 protocols using bms603hs
1
Murine Cytokine ELISA Protocol
2
Immunoglobulin Levels and Autoantibodies in Aging Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sera from 3- and 8-mo-old mice were analyzed with ELISA kits for the measurement of total IgG, IgM, and IgA (88-50400, 88-50470, and 88-50450; all from eBioscience). For IL-6 measurement, we used a high-sensitivity ELISA kit for the measurement of sera from 1-, 2-, and 3-mo-old mice (BMS603HS; eBioscience) and otherwise used an IL-6 ELISA kit (M600B; R&D Systems). The titers of ANA in the sera of 8-mo-old mice were analyzed with a specific ELISA kit (MBS731183; MyBioSource).
3
Serum Inflammation Marker Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum concentrations for the following inflammation markers were determined using ELISA following the manufacturer’s instructions: Serum Amyloid A (SAA, Tridelta, TP802-M), Lipopolysaccharide Binding Protein (LBP, Cell Sciences, CKM043), Interleukin-6 (IL-6, eBioscience, BMS603HS) and tumor necrosis factor-alpha (TNF-α, eBioscience, BMS607HS).
4
Quantification of Inflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5
Serum Cytokine Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum was collected from whole blood samples by centrifugation (1,250 x g, 10 min, room temperature). The serum levels of IL-1β (cat. no. E-EL-M0037c; Elabscience Biotechnology, Inc.), TNF-α (cat. no. E03T0008; BlueGene Biotechnology Co., Ltd.) and IL-6 (cat. no. BMS603HS; Thermo Fisher Scientific, Inc.) were detected using mouse enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's protocol.
6
Inflammatory Cytokine Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the quantification of inflammatory cytokine, tissue lysates were analyzed with mouse IL-6 (Thermo Fisher BMS603HS), IL-1β (Thermo Fisher 88-5019-22), and TNF-α (Thermo Fisher BMS607-3) ELISA kits according to the manufacturer’s instructions. The sample concentrations were determined by comparison with the standard curve.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!