Abe223

Manufactured by Merck Group

Sourced in China

The ABE223 is a laboratory instrument designed for the analysis and measurement of various samples. It utilizes advanced technology to provide accurate and reliable data. The core function of the ABE223 is to perform specific analytical tasks, but its intended use should not be extrapolated beyond the factual information provided.

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Lab products found in correlation

Magna chip g tissue kit, by Merck Group (1 mentions) Ab3350, by Abcam (1 mentions) Ab10812, by Abcam (1 mentions) Zymo spin chip kit, by Zymo Research (1 mentions) Chemidoc mp imager, by Bio-Rad (1 mentions) Epiquik histone h3 modification multiplex assay kit, by Epigentek (1 mentions) Acetyl histone h3 lys14, by Merck Group (1 mentions) Ab18521, by Abcam (1 mentions) Ab17036, by Abcam (1 mentions) Acetyl histone h3 lys23, by Merck Group (1 mentions)

2 protocols using abe223

Chromatin Immunoprecipitation of Acetylated Histones

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Hippocampal tissues were treated using the Magna CHIPTM G Tissue kit (Millipore, Darmstadt, Germany) according to the manufacturer's protocol and sonicated using a Biorupter Pico instrument (Diageuode, Japan) for 12.5 min (45 s, on; 30 s, off; 10 cycles). Cell samples were treated using the Zymo-Spin™ ChIP Kit (ZymoResearch, Irvine, CA) according to the manufacturer's protocol and sonicated for 10 min (30 s, on; 30 s, off; 10 cycles). Cross-linked chromatin was immunoprecipitated with antibodies against acetylated chromatin: anti-H3K9 (ab10812; Abcam, Hong Kong, China), anti-H3K27 (ab3350; Abcam), and anti-H3k4 (#ABE223; Millipore); normal rabbit IgG-B (sc-2763; Santa Cruz Biotechnology, Dallas, TX) was used as a negative control antibody. Three pairs of primers specific for the Asct2 promoter region (see Figure 4G) were used to detect the acetylation status of H3K9, H3K4, and H3K27. Real-time PCR was performed in triplicate.

Wang J., Zhang K., Chen X., Liu X., Teng H., Zhao M, & Sun Z. (2017). Epigenetic Activation of ASCT2 in the Hippocampus Contributes to Depression-Like Behavior by Regulating D-Serine in Mice. Frontiers in Molecular Neuroscience, 10, 139.

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Quantifying Histone Modifications via Immunoblot and ELISA

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Extracted histones were subjected to immunoblot analysis for H3/H4 acetylation and methylation marks using antibodies to acetyl-Histone H3 Lys4 (ABE223; Millipore), acetyl-Histone H3 Lys14 (04-1044; Millipore), acetyl-Histone H3 Lys18 (AB1191; Abcam), acetyl-Histone H3 Lys23 (07-360; Millipore), acetyl-Histone H4 Lys5 (07-327; Millipore), acetyl-Histone H4 Lys8 (61103; Active Motif), acetyl-Histone H4 Lys12 (07-595; Millipore), acetyl-Histone H4 Lys16 (07-329; Millipore), monomethyl-Histone H3 Lys4 (07-436; Millipore), monomethyl-Histone H3 Lys9 (ABE101-S; Millipore), unmethylated-Histone H3 Lys9 (MABE263; Millipore), monomethyl-Histone H3 Lys27 (07-448; Millipore), total Histone H3 (AB18521; Abcam) and total Histone H4 (AB17036; Abcam). Each measured protein was normalized to total H3 or H4. The chemiluminescent blots were imaged with a ChemiDoc MP imager (Bio-Rad) and quantified using Image software (NIH, Bethesda, MD). At the same time, an EpiQuik Histone H3 Modification Multiplex Assay Kit (P-3100; EpiGentek) was used to detect and quantify various H3 methylations simultaneously in an ELISA-like format following the manufacturer’s recommended protocols.

Ge Y., Zhu J., Wang X., Zheng N., Tu C., Qu J, & Ren X. (2018). Mapping dynamic histone modification patterns during arsenic-induced malignant transformation of human bladder cells. Toxicology and applied pharmacology, 355, 164-173.

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