RNA was isolated using the miRVana kit (Life technologies). The Maxima cDNA synthesis kit (Thermofisher Scientific) was used to generate cDNA and 2 µl cDNA was used for Taqman based quantitative real-time mRNA analysis containing 10 µl Taqman Fast Universal polymerase chain reaction (PCR) master mix (2×) no AmpErase UNG (Life Technologies), 1 µl Taqman primer probe (20×), in up to 20 µl nuclease-free water. GAPDH was used as the normalizer and one-way ANOVA was used to perform statistical analysis. Assay IDs were as follows: Hs01113624_g1 [TNFα], Hs00900055_m1 [VEGFA], Hs00985639_m1 [IL-6], Hs01028901_g1 [NFκB2] (Applied Biosystems, Carlsbad, CA). High capacity cDNA reverse transcription kit was used to generate cDNA for miRNAs. One microliter cDNA was used for Taqman based quantitative real-time mRNA analysis containing 5 µl Taqman Universal PCR master mix (2×) no AmpErase UNG (Life Technologies), 0.5 µl TaqMan microRNA assay (Assay ID 002182, Applied Biosystems), in up to 10 µl nuclease-free water. U6 snRNA (Assay ID 001973) for cellular or miR-223 (Assay ID 000526) for sEVs was used for normalization.
Hs01113624 g1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Hs01113624_g1 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It serves as a tool for conducting specific analyses or experiments within a controlled laboratory environment. The core function of this product is to provide a reliable and consistent means of data collection and measurement, though its exact intended use may vary depending on the specific application or research needs.
Automatically generated - may contain errors
Lab products found in correlation
Taqman universal pcr master mix, by Thermo Fisher Scientific (2 mentions)
Hs00900055 m1, by Thermo Fisher Scientific (1 mentions)
Maxima cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Mirvana kit, by Thermo Fisher Scientific (1 mentions)
Amperase ung, by Thermo Fisher Scientific (1 mentions)
Taqman microrna assay, by Thermo Fisher Scientific (1 mentions)
Lhc 9, by Thermo Fisher Scientific (1 mentions)
Purecol collagen, by Advanced BioMatrix (1 mentions)
Il 1α and il 1β duoset, by R&D Systems (1 mentions)
Hs00174092 m1, by Thermo Fisher Scientific (1 mentions)
Hs01555410 m1, by Thermo Fisher Scientific (1 mentions)
High capacity cdna archive kit, by Thermo Fisher Scientific (1 mentions)
Gapdh hs02758991 g1, by Thermo Fisher Scientific (1 mentions)
Nukleospin rna plus, by Macherey-Nagel (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Cell culture plate, by Corning (1 mentions)
2 protocols using hs01113624 g1
1
Quantitative Analysis of Gene and miRNA Expression
2
Cell Culture Reagents and Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell culture medium LHC-9 and DMEM:F12, and Trypsin-EDTA, were bought from Gibco, Thermo Fisher Scientific, Waltham, MA USA. The cell culture flasks were obtained from Nunc A/S, Roskilde, Denmark, while the cell culture plates were from Corning, NY 14,831 USA. PureCol™ collagen was from Advanced BioMatrix, Inc, CA, USA.The ELISA cytoset for CXCL8 was purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, while IL-1α and IL-1β DuoSet were from R&D Systems, Inc, Minneapolis, MN, USA. Cytotoxicity Detection Kit (lactate dehydrogenase; LDH) was obtained from Merck KGaA, Darmstadt, Germany. The RNA isolating kit; NukleoSpin RNA plus, was bought from MACHEREY-NAGEL, Duren, Germany. CH22319 and N-acetyl cystein (NAC) were bought from Merck KGaA, Darmstadt, Germany. High Capacity cDNA Archive Kit, TaqMan Universal PCR Mastermix, TaqMan Gene Expression Assays (CXCL8, Hs00174103_m1, IL-1α; Hs00174092_m1, IL-1β; Hs01555410_m1, TNF-α; Hs01113624_g1, HO-1; Hs01110250_m1, CYP1A1; Hs01054797_g1, CYP1B1; Hs02382916_s1, PAI-2 ; Hs01010736_m1, COX2; Hs00153133_m1 and GAPDH; Hs02758991_g1) were bought from Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA. Other chemicals were purchased from commercial sources at the highest purity available.
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The cell culture medium LHC-9 and DMEM:F12, and Trypsin-EDTA, were bought from Gibco, Thermo Fisher Scientific, Waltham, MA USA. The cell culture flasks were obtained from Nunc A/S, Roskilde, Denmark, while the cell culture plates were from Corning, NY 14,831 USA. PureCol™ collagen was from Advanced BioMatrix, Inc, CA, USA.The ELISA cytoset for CXCL8 was purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, while IL-1α and IL-1β DuoSet were from R&D Systems, Inc, Minneapolis, MN, USA. Cytotoxicity Detection Kit (lactate dehydrogenase; LDH) was obtained from Merck KGaA, Darmstadt, Germany. The RNA isolating kit; NukleoSpin RNA plus, was bought from MACHEREY-NAGEL, Duren, Germany. CH22319 and N-acetyl cystein (NAC) were bought from Merck KGaA, Darmstadt, Germany. High Capacity cDNA Archive Kit, TaqMan Universal PCR Mastermix, TaqMan Gene Expression Assays (CXCL8, Hs00174103_m1, IL-1α; Hs00174092_m1, IL-1β; Hs01555410_m1, TNF-α; Hs01113624_g1, HO-1; Hs01110250_m1, CYP1A1; Hs01054797_g1, CYP1B1; Hs02382916_s1, PAI-2 ; Hs01010736_m1, COX2; Hs00153133_m1 and GAPDH; Hs02758991_g1) were bought from Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA. Other chemicals were purchased from commercial sources at the highest purity available.
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