N-acetyl-L-cysteine (NAC, HY-B0215) was purchased from MedChemExpress (Monmouth, NJ, USA). Annexin V-FITC and PI kit apoptosis detection kit was obtained from BD (Franklin Lakes, NJ, USA). 2',7'-dichlorofluorescin diacetate (DCF-DA) was acquired from Beyotime (Shanghai, China). The following antibodies were used for the western blot analysis: α-tubulin (ab52866, Abcam, Cambridge, UK), GAPDH (60004-1-Ig, Proteintech, Wuhan, China), ENO2 (10149-1-AP proteintech, Wuhan, China), STAT1 (10144-2-AP, Proteintech), PARP1 (13371-1-AP, Proteintech), and H2AX (10856-1-AP, Proteintech).
H2ax 10856 1 ap
Manufactured by Proteintech
Sourced in China
H2AX (10856-1-AP) is a primary antibody produced by Proteintech. It is used to detect the phosphorylated form of the H2AX histone protein, which is a marker of DNA double-strand breaks.
Automatically generated - may contain errors
Lab products found in correlation
2 7 dichlorofluorescin diacetate dcf da, by Beyotime (1 mentions)
Stat1 10144 2 ap, by Proteintech (1 mentions)
α tubulin ab52866, by Abcam (1 mentions)
Gapdh 60004 1 ig, by Proteintech (1 mentions)
Parp1 13371 1 ap, by Proteintech (1 mentions)
Curcumin, by MedChemExpress (1 mentions)
β actin 66009 1 ig, by Proteintech (1 mentions)
Dpph free radical scavenging capacity assay kit, by Solarbio (1 mentions)
Abts free radical scavenging capacity assay kit, by Solarbio (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Calcein am, by Beyotime (1 mentions)
Cck 8 cell proliferation and cytotoxicity assay kit, by Solarbio (1 mentions)
Total superoxide dismutase assay kit with wst 8, by Beyotime (1 mentions)
Lipid peroxidation mda assay kit, by Beyotime (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Ethanol c2h5oh, by Sinopharm (1 mentions)
2 protocols using h2ax 10856 1 ap
1
NAC-Induced Apoptosis and Oxidative Stress
2
Antioxidant and Anti-Inflammatory Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Polyethyleneimine (PEI, M.W. 10,000, 99%), manganese chloride tetrahydrate (MnCl2·4H2O), and 3,3′,5,5’-Tetramethylbenzidine (TMB) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl), sodium acetate (NaAc), acetic acid (HAc), and ethanol (C2H5OH) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Titanium (IV) sulfate (Ti(SO4)2) was provided by Macklin (Shanghai, China). All the chemicals were of analytical grade and used directly without further purification.
Curcumin was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Lipopolysaccharide (LPS) was provided by Sigma Aldrich (St. Louis, MO, USA). TNF-α (#ab1793), iNOS (#ab178945) and IL-10 (#ab9969) were obtained from Abcam (Cambridge, MA, USA). H2AX (#10856-1-AP) and β-actin (#66009-1-Ig) were obtained from Proteintech (Wuhan, China). Calcein AM, Propidium Iodide, the Total Superoxide Dismutase Assay Kit with WST-8, the Reactive Oxygen Species Assay Kit, and the Lipid Peroxidation MDA Assay Kit were obtained from Beyotime (Shanghai, China). The ABTS Free Radical Scavenging Capacity Assay Kit, DPPH Free Radical Scavenging Capacity Assay Kit, and CCK-8 Cell Proliferation and Cytotoxicity Assay Kit were purchased from Solarbio (Beijing, China).
Curcumin was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Lipopolysaccharide (LPS) was provided by Sigma Aldrich (St. Louis, MO, USA). TNF-α (#ab1793), iNOS (#ab178945) and IL-10 (#ab9969) were obtained from Abcam (Cambridge, MA, USA). H2AX (#10856-1-AP) and β-actin (#66009-1-Ig) were obtained from Proteintech (Wuhan, China). Calcein AM, Propidium Iodide, the Total Superoxide Dismutase Assay Kit with WST-8, the Reactive Oxygen Species Assay Kit, and the Lipid Peroxidation MDA Assay Kit were obtained from Beyotime (Shanghai, China). The ABTS Free Radical Scavenging Capacity Assay Kit, DPPH Free Radical Scavenging Capacity Assay Kit, and CCK-8 Cell Proliferation and Cytotoxicity Assay Kit were purchased from Solarbio (Beijing, China).
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