L. enzymogenes YC36 was grown in 40% TSB medium supplemented with different concentrations of NaCl and indole (Solarbio) for 2 days and grown at different times. Then, the liquid of bacteria was centrifuged. The supernatant was vacuum‐concentrated and dissolved in approximately 2 ml of methanol. Afterward, the liquid was concentrated again and dissolved in 0.5 ml of methanol and 1 ml of DMSO. Finally, an aliquot (5 μl) of each crude extract was analyzed using HPLC (Thermo Scientific Dionex Ultimate 3000 instrument; YMC‐Pack Pro C18 250 × 4.6 mm, S‐5 m, 12 nm). indole was not added to the Ctrl group. For semiquantification, the peak areas of HSAF and its analogs were measured to obtain the relative yields of the compounds. The following chromatography conditions were used: solvent A: water containing 0.1% formic acid; solvent B: acetonitrile containing 0.1% formic acid; flow rate: 1 ml/min; detection wavelength: 320 nm; and solvent gradient from 20% B to 35% in the first 5 min, increased to 75% at 12 min, to 90% at 20 min, to 100% at 27 min, followed by 4 min with 100% B.
Indole
Manufactured by Solarbio
Sourced in China
Indole is a heterocyclic organic compound. It is a colorless solid with a slightly unpleasant odor. Indole is commonly used as a chemical building block in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Decanal, by Merck Group (1 mentions)
2 hexenal, by Merck Group (1 mentions)
Cis 3 hexenyl hexanoate, by Maclin Biochemical Technology (1 mentions)
Benzyl nitrile, by Merck Group (1 mentions)
Caffeine, by ChemFaces (1 mentions)
Geraniol, by Yuanye Bio-Technology (1 mentions)
Phenylacetaldehyde, by Yuanye Bio-Technology (1 mentions)
1 octen 3 ol, by Yuanye Bio-Technology (1 mentions)
δ cadinene, by Yuanye Bio-Technology (1 mentions)
Z 3 hexenyl acetate, by Merck Group (1 mentions)
α terpineol, by Yuanye Bio-Technology (1 mentions)
2 heptanol, by Merck Group (1 mentions)
Benzaldehyde, by Maclin Biochemical Technology (1 mentions)
Methyl salicylate, by Yuanye Bio-Technology (1 mentions)
Nerolidol, by Yuanye Bio-Technology (1 mentions)
Linalool oxide, by Yuanye Bio-Technology (1 mentions)
Guaiacol, by Maclin Biochemical Technology (1 mentions)
2 protocols using indole
1
HSAF Production by L. enzymogenes YC36
Check if the same lab product or an alternative is used in the 5 most similar protocols
L. enzymogenes YC36 was grown in 40% TSB medium supplemented with different concentrations of NaCl and indole (Solarbio) for 2 days and grown at different times. Then, the liquid of bacteria was centrifuged. The supernatant was vacuum‐concentrated and dissolved in approximately 2 ml of methanol. Afterward, the liquid was concentrated again and dissolved in 0.5 ml of methanol and 1 ml of DMSO. Finally, an aliquot (5 μl) of each crude extract was analyzed using HPLC (Thermo Scientific Dionex Ultimate 3000 instrument; YMC‐Pack Pro C18 250 × 4.6 mm, S‐5 m, 12 nm). indole was not added to the Ctrl group. For semiquantification, the peak areas of HSAF and its analogs were measured to obtain the relative yields of the compounds. The following chromatography conditions were used: solvent A: water containing 0.1% formic acid; solvent B: acetonitrile containing 0.1% formic acid; flow rate: 1 ml/min; detection wavelength: 320 nm; and solvent gradient from 20% B to 35% in the first 5 min, increased to 75% at 12 min, to 90% at 20 min, to 100% at 27 min, followed by 4 min with 100% B.
+ Expand
L. enzymogenes YC36 was grown in 40% TSB medium supplemented with different concentrations of NaCl and indole (Solarbio) for 2 days and grown at different times. Then, the liquid of bacteria was centrifuged. The supernatant was vacuum‐concentrated and dissolved in approximately 2 ml of methanol. Afterward, the liquid was concentrated again and dissolved in 0.5 ml of methanol and 1 ml of DMSO. Finally, an aliquot (5 μl) of each crude extract was analyzed using HPLC (Thermo Scientific Dionex Ultimate 3000 instrument; YMC‐Pack Pro C18 250 × 4.6 mm, S‐5 m, 12 nm). indole was not added to the Ctrl group. For semiquantification, the peak areas of HSAF and its analogs were measured to obtain the relative yields of the compounds. The following chromatography conditions were used: solvent A: water containing 0.1% formic acid; solvent B: acetonitrile containing 0.1% formic acid; flow rate: 1 ml/min; detection wavelength: 320 nm; and solvent gradient from 20% B to 35% in the first 5 min, increased to 75% at 12 min, to 90% at 20 min, to 100% at 27 min, followed by 4 min with 100% B.
2
Comprehensive Chemical Sourcing for Analytical Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anhydrous
sodium sulfate (AR grade) was purchased from GHTECH (Shantou, Guangdong,
China); dichloromethane (AR grade) was ordered from PN-CHEM (Zhengzhou,
Henan, China). Tridecanol, 2-hexenal, 2-heptanol, (Z)-3-hexenyl acetate,
decanal, and benzyl nitrile were purchased from Sigma (St. Louis,
Missouri, USA); α-farnesene, cis-3-hexenyl hexanoate, 1-hexanol,
(Z)-3-hexenol, benzaldehyde, phytol acetate, ethyl decanoate, 3-octanol,
and guaiacol were obtained from Macklin (Shanghai, China); β-ocimene,
α-copaene, germacrene D, and neophytadiene were purchased from
J&K Chemicals (Shanghai, China); cis-jasmone, caffeine, and (E)-2-hexenol
were obtained from ChemFaces (Wuhan, China); indole was purchased
from Solarbio (Beijing, China); benzyl alcohol, linalool, geraniol,
nerol, nerolidol, α-terpinene, α-humulene, hexadecanoic
acid methyl ester, phytol, phenylacetaldehyde, α-terpineol,
2-phenylethanol, 1-octen-3-ol, δ-cadinene, caryophyllene oxide,
neral, methyl salicylate, and linalool oxide were purchased from Yuanye
Bio-Technology Co. (Shanghai, China). The alkane standard (C6–C44) was purchased from AccuStandard (New Haven,
CT, USA).
sodium sulfate (AR grade) was purchased from GHTECH (Shantou, Guangdong,
China); dichloromethane (AR grade) was ordered from PN-CHEM (Zhengzhou,
Henan, China). Tridecanol, 2-hexenal, 2-heptanol, (Z)-3-hexenyl acetate,
decanal, and benzyl nitrile were purchased from Sigma (St. Louis,
Missouri, USA); α-farnesene, cis-3-hexenyl hexanoate, 1-hexanol,
(Z)-3-hexenol, benzaldehyde, phytol acetate, ethyl decanoate, 3-octanol,
and guaiacol were obtained from Macklin (Shanghai, China); β-ocimene,
α-copaene, germacrene D, and neophytadiene were purchased from
J&K Chemicals (Shanghai, China); cis-jasmone, caffeine, and (E)-2-hexenol
were obtained from ChemFaces (Wuhan, China); indole was purchased
from Solarbio (Beijing, China); benzyl alcohol, linalool, geraniol,
nerol, nerolidol, α-terpinene, α-humulene, hexadecanoic
acid methyl ester, phytol, phenylacetaldehyde, α-terpineol,
2-phenylethanol, 1-octen-3-ol, δ-cadinene, caryophyllene oxide,
neral, methyl salicylate, and linalool oxide were purchased from Yuanye
Bio-Technology Co. (Shanghai, China). The alkane standard (C6–C44) was purchased from AccuStandard (New Haven,
CT, USA).
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