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Anti pd l1 monoclonal antibody 29e 2a3

Manufactured by BioLegend

The Anti-PD-L1 monoclonal antibody (29E.2A3) is a laboratory reagent produced by BioLegend. It is designed to bind to the PD-L1 protein, which is a key regulator of immune responses.

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2 protocols using anti pd l1 monoclonal antibody 29e 2a3

1

Immunoblotting of S100A9 and PD-L1

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Antibodies against S100A9 (PA5-19,075, 1:1000) and Flag (PA1-984B, 1:1000) were obtained from Thermo Fisher, and anti-GAPDH antibodies (#5174, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA). The anti-PD-L1 monoclonal antibody (29E.2A3) was obtained from BioLegend (San Diego, CA). Allophycocyanin (APC)-conjugated anti-mouse IgG antibodies (115–605-166) were obtained from Jackson ImmunoResearch Inc. (West Grove, PA). Human S100A9 recombinant proteins were obtained from R&D (9254-S9-050, R&D systems, Minneapolis, MN), and human S100A8/S100A9 heterodimer proteins were from biolegend (753,406, BioLegend, San Diego, CA).
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2

Antibody Detection for S100A9, Flag, GAPDH, and PD-L1

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Antibodies against S100A9 (PA5-19075, 1:1000) and Flag (PA1-984B, 1:1000) were obtained from Thermo Fisher, and anti-GAPDH antibodies (#5174, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA). The anti-PD-L1 monoclonal antibody (29E.2A3) was obtained from BioLegend (San Diego, CA). Allophycocyanin (APC)-conjugated anti-mouse IgG antibodies (115-605-166) were obtained from Jackson ImmunoResearch Inc. (West Grove, PA).
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