Sigmastat 3

Vessel diameter and Ca²⁺_i responses with EGTA, nifedipine, and mibefradil vs. normal medium were analysed using ANOVA followed by the student–Newman–Keuls post hoc test in SigmaStat 3.1. Diameter and Ca²⁺_i responses in Notch3^−/− vs. wild-type and thapsigargin Ca²⁺_i responses vs. normal medium were analysed using student’s t-test in SigmaStat 3.1. Gene expression was analysed using student’s t-test and Bonferroni’s correction. The genes were ordered after significance (Table A1), and the difference between the groups was expressed as Log2 fold-change (Log2FC). p < 0.05 was considered statistically significant. All values are expressed as means ± SEM.

Data are given as population mean ± SEM from 8 hearts prior to Lugol exposure (Tyrode) and 7 hearts after Lugol exposure (Lugol) in contracting hearts or from 4 hearts in the optical mapping study. Statistical analysis was performed with Sigmastat 3.5. Following data normality testing, either the paired Student's t-test or the non-parametric equivalent, Wilcoxon signed rank test, was performed on paired data from 7 contracting hearts, using the mean of each parameter in each condition (Tyrode vs. Lugol). Statistical significance was taken as p < 0.05.
Alternative analysis where n was individual paired stretches (with the 1^st 2^nd and 3^rd stretch in each Tyrode sequence paired with the equivalent Lugol stretch in each heart) resulted in the same statistical outcomes with respect to p < or >0.05.
For pressure and AP parameters the effects and possible interactions of stretch and Lugol exposure were tested by 2 way analysis of variance (2 way ANOVA) with balloon volume (stretch) and exposure to Lugol as the 2 factors. The parameter means from each heart in each condition were used. A result of p < 0.05 for either factor or factor interaction was followed by Holm-Sidak multiple pairwise comparision tests.

A two-way ANOVA test was used to determine the statistically significant differences in SBS surveying. The data of the flexural modulus and degree of conversion were statistically analyzed using one-way ANOVA and Tukey’s test. Such tests were performed after all data had passed a Shapiro-Wilk normality test (p > 0.05). Analyses were performed using SigmaStat 3.5 statistical software (Systat, San Jose, CA, USA). The level of significance was considered to be 5%.

The serum Ca and P levels were expressed as mean and standard deviation. Independent samples were analyzed by analysis of variance (ANOVA), followed by the multiple comparison Tukey test for symmetric distribution, adjusting general linear models with Gamma distribution, followed by the multiple comparison Wald test for asymmetric data. Pearson's correlation was performed between the Ca area under the curve (AUC) and the serum levels of 25-hydroxyvitamin D, and between the Ca AUC and the age of the women. All analyses were performed by the statistical applications SAS for Windows^® version 9.3 and SigmaStat 3.5. The significance level was set at 5%.

The SigmaStat 3.5 (Point Richmond, CA, USA) software package was used to perform statistical evaluation and significance was reached at P = 0.05. Due to the small number of sampling, all the data are presented as median and IQR independently of their distribution.
Effect of time on PR and RR was tested with one way ANOVA test in RANK for repeated measures. Spearman rank order test was used to test: 1) correlations between quality of sedation, induction and recovery (score and VAS) in all horses (n = 28) and in the horses that did not receive any additional drug during the procedure (n = 20); 2) correlation between time at which clinical signs of lightening of anaesthesia appeared and duration of anaesthesia; 3) correlation between T_movement and predicted plasma concentrations of S-ketamine at T_movement in all horses that did not move before the end of surgery; 4) correlation between T_movement and the area under the curves of the predicted concentration of S-ketamine against time from 2 to 20 minutes (AUC S-k _2–20).
Differences of measured S-ketamine plasma concentrations at 2, 4, 8 minutes after administration between the horses receiving one or two boluses were tested by a 2 way-ANOVA test for repeated measures.