A 96-well culture plate (3599, Corning, USA) was pre-coated by 10 μg cm−2 collagens, including B, P5BP5, P10BP10, P10BBP10 and Type I collagen (C7661, SIGMA, USA), at 4 °C overnight. Then the sample solution was removed and the plate was dried in the air for 12 h. After 2 h ultraviolet sterilization, the plate was blocked by 5% BSA (A600332-0100, Sangon Biotech, China) for 2 h. Then 2000 MC-3T3 cells were seeded into the wells and Cell Counting Kit-8 (BA00208, BIOSS, China) was used to detect the cell proliferation according to the manufacturer’s instructions. At days 0, 1, 3, and 5, 10 μl Cell Counting Kit-8 solution was added into each well and the absorbance at 450 nm was measured through SYNER GY H1 (BioTek, USA). The culture medium contains 90% α-MEM medium (SH30265.01, Hyclone, USA), 10% fetal bovine serum (FND500, ExCell, China), and 1% Penicillin–Streptomycin (KGY0023, KeyGEN, China). The culture medium was renewed every two days.
Cell counting kit 8 (cck8)
Manufactured by Bioss Antibodies
Sourced in China
The Cell Counting Kit-8 is a colorimetric assay used to determine the number of viable cells in a cell proliferation or cytotoxicity assay. It utilizes a highly water-soluble tetrazolium salt to measure the metabolic activity of cells.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (3 mentions)
Synergy h1, by Agilent Technologies (2 mentions)
C7661, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Agilent Technologies (1 mentions)
Kgy0023, by Keygen Biotech (1 mentions)
Multiskan skyhigh, by Thermo Fisher Scientific (1 mentions)
Annexin 5 apc and 7 aad, by BioLegend (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Rnase a, by Solarbio (1 mentions)
Axio vert a1, by Zeiss (1 mentions)
Spectramax 190, by Molecular Devices (1 mentions)
C11875500bt, by Thermo Fisher Scientific (1 mentions)
Microplate reader, by Tecan (1 mentions)
Ethyl acetate, by Sinopharm (1 mentions)
Dapi staining, by Wuhan Servicebio Technology (1 mentions)
Methacrylic anhydride, by Maclin Biochemical Technology (1 mentions)
ε caprolactone, by Maclin Biochemical Technology (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
20 protocols using cell counting kit 8 (cck8)
1
Cell Proliferation on Collagen Coatings
2
Cell Proliferation Evaluation via CCK8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation activity was evaluated via Cell Counting Kit-8 assay (CCK8, Bioss, China). At different time points, the medium was replaced with 100 μl of fresh medium containing 10 μl of CCK8. Three hours after the addition of CCK8, cell viability was determined with a microplate reader (Thermo Electron, USA) at a wavelength of 450 nm. All plates had control wells containing medium without cells to obtain a value for background luminescence, which was subtracted from the test sample readings. Each experiment was performed in triplicate. The growth kinetics were performed by the OD values (y axis) and day times (x axis).
3
Cell Proliferation Analysis using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was analysed using Cell Counting Kit‐8 (CCK‐8; Bioss, Beijing, China). See Supporting Information for details.
4
Cell Proliferation Analysis using CCK-8 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was analyzed using the Cell Counting Kit-8 (BIOSS, Beijing, China) according to the manufacturer’s protocol. The melanoma cells transfected with siRNA expressing HLA-DQA1 and empty vector were diluted and seeded at a density of 1 × 103 cells per well in a 96-well plate containing 0.1 mL of culture medium. After 24 h of incubation, each well was treated with 10 μl of CCK-8 solution at 37 °C. Subsequently, the absorbance at 450 nm was measured using the Multiskan SkyHigh (Thermo Fisher, Stony Creek, the US).
5
Cell Proliferation Assay for LUAD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the operating manual of the kit, we applied the Cell Counting Kit-8 (CCK-8, Bioss, China) to detect the growth activity of various LUAD cell lines. An enzyme-linked immuno-absorbance assay (BioTek, USA) was used to reflect the cell numbers by measuring the absorbance at 450 nm during cell growth.
6
Cell Cytotoxicity Assay of Mzb and CDDP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cytotoxicity assays were performed using a Cell Counting Kit-8 (CCK-8, Bioss Inc., China) following the manufacturer’s instructions. Cells in the logarithmic growth phase were used for experiments. Cells were seeded in 96-well plates at a density of 5 × 103 per well (200 μL per well) and cultured for 24 h at 37°C. Cells were either allowed to grow in medium alone or in medium containing increasing concentrations of Mzb, CDDP, or a combination of the two drugs, and each group was prepared with 5 replicate wells. Seventy-two hours later, the cells were observed and photographed by optical microscopy. Then, 20 μL of CCK-8 working solution was added to each well, and the cells were incubated at 37°C and 5% CO2 for 2 hours. The absorbance of each well was measured at 450 nm with a microplate reader and the cell viability curve was plotted.
7
RMC Proliferation Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RMC proliferation was assessed according to the instructions provided by Cell Counting Kit-8 (Bioss, Beijing, China). Briefly, RMCs were collected and transferred into 96-well microplates, incubated with 100 μL of CCK8 solution for 4 h, and then the absorbance at 450 nm was recorded.
8
Cell Proliferation Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was assessed according to instructions provided with a Cell Counting Kit-8 (Bioss, Beijing, China). Briefly, cells were collected and transferred into 96-well flat-bottomed microplates. Next, the cells were incubated with 100 μl of CCK8 solution for 4 h, after which, the absorbance at 450 nm was recorded. We did three replications of the CCK8 experiment. The cell viability was calculated as follows: Cell viability = [(OD value of treatment group—OD value of blank group)/(OD value of control group—OD value of blank group)] × 100%.
9
Cell viability and apoptosis assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells cultured in 96-well plates (1500 cells/well) were treated with different concentrations of UA for various times before subjected to Cell Counting Kit-8 (CCK-8, Bioss, MA, USA) assay to determine cell viability. For cell cycle analysis, cells were fixed in 75% ethanol. After washing with PBS, cells were stained by propidium iodide (PI, MedChemExpress, NJ, USA) in staining solution supplemented with 0.2% Triton X-100 (Solarbio, Beijing, China) and RNase A (Solarbio). Cell cycle was assessed using a flow cytometer (BD FACSCelesta, OA, USA). For cell death detection, cell samples were incubated with 0.5 μL APC-Annexin V and 7-AAD (BioLegend, CA, USA). The number of apoptotic cells in 1 × 105 cells was counted by a flow cytometer (BD FACSCelesta).
10
BEAS-2B Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The viability of BEAS-2B cells incubated for 48 h at different concentrations, including 0, 2.5, 5, 10, 20, 40, 80, 160 and 320 μg/mL, was assessed using a Cell Counting Kit-8 (Bioss, China). A total of 5000 cells in 100 μL of complete medium (DMEM + 10% FBS) were added to the wells of a 96-well plate. The CCK-8 reagent (10 μL) was diluted with DMEM at a 1: 10, and 100 μL of the working solution was added to the wells. Absorbance values were measured at 450 nm after 2 h of incubation. For the proliferation assay, three replicates were treated with the above treatments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!