Isoniazid

Manufactured by Maclin Biochemical Technology

Sourced in China

Isoniazid is a laboratory reagent used in biochemical and analytical applications. It is a chemical compound with the formula C₆H₇N₃O. Isoniazid functions as a precursor and intermediate in various chemical syntheses and analyses.

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Lab products found in correlation

Kanamycin, by Merck Group (1 mentions) Hygromycin, by Roche (1 mentions) Streptomycin, by Maclin Biochemical Technology (1 mentions) Rifampin, by Maclin Biochemical Technology (1 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt abts, by Merck Group (1 mentions) Nicotinic acid, by Maclin Biochemical Technology (1 mentions) Phloretin, by Yuanye Bio-Technology (1 mentions) 1 1 diphenyl 2 picrylhydrazyl dpph, by Maclin Biochemical Technology (1 mentions) Phloretin, by Maclin Biochemical Technology (1 mentions)

2 protocols using isoniazid

TB47 Formulation and Antimicrobial Drug Preparation

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TB47 was synthesized in a batch and supplied by Guangzhou Eggbio Co. Ltd with purity 98.67% by High Performance Liquid Chromatograph (Batch number: TB47160616). It was formulated in 0.05% CMC-Na (sodium salt of carboxy methyl cellulose) for in vivo study. Rifampin and streptomycin were both bought from TCI (Japan) and dissolved in distilled water for in vivo use. streptomycin and isoniazid (Macklin, Shanghai) were dissolved in distilled water, and Rifampin and TB47 were dissolved in dimethyl sulfoxide (DMSO) for in vitro use. All drugs prepared weekly for in vivo studies. Hygromycin was bought from Roche (Swiss) in liquid form and kanamycin was bought from Sigma (USA). They were dissolved in distilled water for in vitro study.

Liu Y., Gao Y., Liu J., Tan Y., Liu Z., Chhotaray C., Jiang H., Lu Z., Chiwala G., Wang S., Makafe G., Islam M.M., Hameed H.M., Cai X., Wang C., Li X., Tan S, & Zhang T. (2019). The compound TB47 is highly bactericidal against Mycobacterium ulcerans in a Buruli ulcer mouse model. Nature Communications, 10, 524.

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Antioxidant and Cytotoxicity Evaluation

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Phloretin (Phl, purity ≥ 98%), isoniazid (Inz, purity = 98%), 2-picolinic acid (99%), theophylline (99%), 4-aminobenzamide (98%), proline (99%), nicotinic acid (99%) and 4-picolinic acid (99%) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Phloretin (HPLC ≥ 98%) and maleic (99%) acid were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was purchased from Sigma Life Science (St. Louis, MO, USA). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Methanol was obtained from Guangzhou Reagent Co., Ltd. (Guangzhou, China). The A549 cell line and HepG2 cell line were purchased from the Institute of Biochemistry and Cell Biology Sciences, Chinese Academy of Sciences (Shanghai, China).

Lu Z., Chen H., Mo J., Yuan X., Wang D., Zheng X, & Zhu W. (2023). Cocrystal of phloretin with isoniazid: preparation, characterization, and evaluation. RSC Advances, 13(16), 10914-10922.

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