1 1 diphenyl 2 picrylhydrazyl dpph folin ciocalteau reagent

Soybean (Glycine max L.) seeds of cultivar ‘Sowonkong’ were purchased from a local store in Deagu, Korea. The cultivar was released as a sprout cultivar in 1999 [51 ]. The mean seed weight was 12 g of 100 seeds. Persimmon fruits of cultivar ‘Sangjudungsi’ were obtained from Sangju Persimmon Experiment Station (Gyeongsangbukdo Agricultural Research and Extention Services, Sangju, Korea). The freeze-dried fruits were ground into powder using a commercial grinder (Speed Rotor Mill, Model KT-02A, Seishin, Fukuoka, Japan) and strained through a 100-mesh sieve. The following chemicals and reagents were obtained for the present study: Indolbi (INB) (Sammi Chemical Industries, Incheon, Korea); 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin-Ciocalteau-reagent, isoflavone standards (≥95% purity, Sigma-Aldrich Corporation, St. Louis, MO, USA), dimethyl sulfoxide (DMSO), and pyrogallol (Sigma-Aldrich Corporation, St. Louis, MO, USA); amino acid standards (Wako Pure Chemical Industries, Ltd., Osaka, Japan). All the other chemicals were of analytical grade.

Soybean (Glycine max L.) cv. Seoritae, a landrace with medium seed size (40 g/100 seeds), was obtained from Nonghyup (Daegu, Korea). Illite powder was obtained from Yeongdong-gun, Chungcheong-bukdo, Korea. The following chemicals and reagents were obtained for the present study: 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin–Ciocalteau reagent, isoflavone standards (≥95% purity, Sigma-Aldrich Corporation, St. Louis, MO, USA), dimethyl sulfoxide (DMSO), and pyrogallol (Sigma-Aldrich Corporation, St. Louis, MO, USA); amino acid standards (Wako Pure Chemical Industries, Ltd., Osaka, Japan). All the other chemicals were of analytical grade.