Nunc plate

Manufactured by Thermo Fisher Scientific

Sourced in United States

NUNC plates are high-quality multiwell plates designed for various laboratory applications. They are manufactured using durable polystyrene and feature a flat bottom, which provides a consistent surface for cell growth and various assays. The plates are available in different well sizes and formats to accommodate different sample volumes and experimental requirements.

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Lab products found in correlation

Pierce bca assay kit, by Thermo Fisher Scientific (6 mentions) Male ab plasma, by Merck Group (3 mentions) Alp conjugated goat anti mouse secondary antibody, by Merck Group (2 mentions) P nitrophenyl phosphate, by Merck Group (2 mentions) Spectramax plus 384, by Molecular Devices (2 mentions) Tmb substrate solution, by Merck Group (2 mentions) Secondary streptavidin hrp, by BD (2 mentions) Orbitrap xl, by Thermo Fisher Scientific (2 mentions) Nanoacquity uplc, by Waters Corporation (2 mentions) Celltiter 96 proliferation assay mts, by Promega (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Genios plate reader, by Tecan (1 mentions) Optima max e, by Beckman Coulter (1 mentions) 0.22 μm filter, by Merck Group (1 mentions) Anti cd28 antibody, by BD (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Magnetic bead, by Miltenyi Biotec (1 mentions) Geltrex, by Thermo Fisher Scientific (1 mentions) Versene edta solution, by Lonza (1 mentions) Stemflex media, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Nunc Maxisorb F96 microtiter plates Nunc Maxisorp flat-bottom clear plates Nunc omnitray plates NUNC™ Maxisorp 384 well plates Nunc MaxSorp ELISA plates Black Nunc™ F96 MicroWell™ Polystyrene Plates Nunc 96-well black optical-bottom plates Nunc MaxiSorp® flat-bottom 96 well ELISA plates Black Nunc MicroWell 96-well Optical Bottom plates Nunc-Immuno Maxisorb™ plates

46 protocols using nunc plate

Biomaterial Cytotoxicity Evaluation

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Biomaterial cytotoxicity was assessed following the ISO 10993-5:2009 (Annex C) norm.
MC3T3-E1 cells (1x10 5 cells cm -2 ) were seeded on 96-well NUNC plates (Thermo Fisher Scientific, Waltham, MA, USA) for 24 h. The materials were also incubated for 24 h in 48-well NUNC plates (Thermo Fisher Scientific) in DMEM with 1% of penicillin/streptomycin and 10% FBS. Then, the cell culture medium was replaced with the medium exposed to the materials followed by an incubation of 24 h. To measure cell viability, the CellTiter 96® Proliferation Assay (MTS) (Promega, Madison, WI) was used according to manufacturer's guidelines. As a negative control, wells with only cells were used. As a positive control, cells were incubated in latex, a compound well known for being cytotoxic. The material was considered cytotoxic when presented cell viability below 70%.

Cerqueira A., Romero-Gavilán F., Araújo-Gomes N., García-Arnáez I., Martinez-Ramos C., Ozturan S., Azkargorta M., Elortza F., Gurruchaga M., Suay J., Goñi I, & Goñi I. (2020). A possible use of melatonin in the dental field: Protein adsorption and in vitro cell response on coated titanium. Materials science & engineering. C, Materials for biological applications, 116.

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Splenocyte Proliferation Assay for β-lg and κ-CN

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For the MTT proliferation assay, splenocytes from naïve mice were seeded on 96-well culture Nunc™ plates at the density of 10⁶/mL in complete medium (CM: RPMI−1640 containing L glutamine and supplemented with 1 mM HEPES, 10 U/mL penicillin-streptomycin, 1 mM sodium pyruvate, 1 mM non-essential amino acids, and 10% heat-inactivated fetal bovine serum (FBS)) and incubated at 37 °C, 5% CO₂, and 90% air humidity. After 12 h of culture stabilization, cells were stimulated with 10, 50, 100, and 200 µg/mL β-lg or κ-CN. Positive control cells were incubated with 10 µg/mL Concanavalin A (Con-A). MTT assay was performed according to the manufacturer’s instructions (Cat. No. 10009365; Cayman Chemical, Ann Arbor, MI, USA). After 120 h of culture, 10 µL of MTT reagent was added to each well. Next, cells were incubated for 4 h, and subsequently, 100 µL of crystal dissolving solution was added. Plates were incubated for another 4 h, and the absorbance was measured at λ = 570 nm using a UVM 340 spectrophotometer (ASYS-Hitech GmbH, Eugendorf, Austria). The lymphocyte proliferation index (PI) was calculated by dividing the absorbance of stimulated cells by the absorbance of unstimulated cells and expressed as a mean of the group (n = 6) ± SD.

Złotkowska D., Stachurska E., Fuc E., Wróblewska B., Mikołajczyk A, & Wasilewska E. (2021). Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies. Nutrients, 13(2), 349.

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Differentiation of THP-1 Monocytes into Macrophages

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Cells were cultured in T-75 vented flasks with complete RPMI 1640 (cRPMI) supplemented with 10% fetal bovine serum (FBS). For differentiation, the THP-1 cells were centrifuged at 1200 rpm for 5 min in a 50 mL tube at 21 °C. After discarding the supernatant, the pellet was re-suspended with the media and counted. Then, THP-1 cells were seeded at a density of 1 × 10⁵ cells/mL in Nunc plates (12, 48 or 96-wells depending on aim of analysis). Cells were differentiated into adherent macrophages for 72 h at 37 °C and 5% CO₂ using 100 nM Phorbol 12-myristate 13-acetate (PMA). Individual plates were set up for each time point during the experiment. To determine multiplicity of infection (MOI), THP-1 cells were also seeded in glass 8-well chamber Nunc Lab-Tek II slides at the same density (Fisher scientific, Dublin, Ireland).

Bahlool A.Z., Fattah S., O’Sullivan A., Cavanagh B., MacLoughlin R., Keane J., O’Sullivan M.P, & Cryan S.A. (2022). Development of Inhalable ATRA-Loaded PLGA Nanoparticles as Host-Directed Immunotherapy against Tuberculosis. Pharmaceutics, 14(8), 1745.

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Amyloid Fibril Formation Kinetics

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Prior to sample preparation, protein stock solutions were centrifuged in an Optima MAX-E ultracentrifuge (Beckman) for 3 to 4 h at 40,000 rpm in order to remove aggregates. Additionally, all assay components were filtered through a 0.22 μm filter (Merck) before the samples were prepared. For all measurements, 200 μl of each sample was incubated in 96-well Nunc plates (Nunc, Thermo Fisher) sealed with Crystal Clear PP sealing foil (HJ-Bioanalytik GmbH). Thioflavin T assays were carried out in triplicates with 15 μM protein, 7.5 μM ThT, 0.05% sodium azide, pH 7.4 or 6.4, at 37 °C under continuous orbital shaking in a Tecan Genios platereader with the shaking intensity set to high (Tecan Group Ltd). For determining the ThT fluorescence of the samples, the excitation wavelength was 440 nm, the emission wavelength was 480 nm, and the gain was set to 70 to 75. Values of midpoint amyloid fibril formation (t₅₀) were determined using a Boltzmann fit.

Rottenaicher G.J., Weber B., Rührnößl F., Kazman P., Absmeier R.M., Hitzenberger M., Zacharias M, & Buchner J. (2021). Molecular mechanism of amyloidogenic mutations in hypervariable regions of antibody light chains. The Journal of Biological Chemistry, 296, 100334.

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Isolation and Activation of Memory T Cells

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Peripheral blood was collected from healthy donors at the Swiss Blood Donation Center of Lugano (Switzerland), with informed consent (authorization number CE 3428 from the Ethical Committee of the Canton Ticino). Peripheral blood mononuclear cells (PBMCs) were isolated through gradient centrifugation (GE Healthcare, Ficoll-Paque Plus) and further enriched for CD4⁺ or CD8⁺ T lymphocytes by positive selection using magnetic beads (Miltenyi Biotec), following the manufacturer’s protocols. Memory T lymphocyte subsets were then sorted on a SORP FACSymphony S6 (BD Biosciences) based on the expression of the following surface markers: CD4⁺ (or CD8⁺) CD25^– CD45RA^– CCR7^+/−. Sorted cells were cultured in RPMI 1640 medium supplemented with 5% human serum, 1% nonessential amino acids, 1% sodium pyruvate, 1% l-glutamine, penicillin, streptomycin, and 50 μM β-mercaptoethanol. T cell activation was performed as previously described (75 (link), 76 (link)) using plate-bound anti-CD3 antibody (0.7 μg/ml; clone TR66, recombinant, made in-house) (77 (link)) and anti-CD28 antibody (1 μg/ml, BD Pharmingen) in 96-well NUNC plates (Thermo Fisher Scientific). After 48 hours, cells were removed from the stimuli and further expanded for up to 10 days in culture. Recombinant interleukin-2 (IL-2) (50 U/ml, made in-house) was added to the cultures starting from day 5.

Cortesi A., Gandolfi F., Arco F., Di Chiaro P., Valli E., Polletti S., Noberini R., Gualdrini F., Attanasio S., Citron F., Ho I.L., Shah R., Yen E.Y., Spinella M.C., Ronzoni S., Rodighiero S., Mitro N., Bonaldi T., Ghisletti S., Monticelli S., Viale A., Diaferia G.R, & Natoli G. (2024). Activation of endogenous retroviruses and induction of viral mimicry by MEK1/2 inhibition in pancreatic cancer. Science Advances, 10(13), eadk5386.

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Microdilution Assay for Nanosilver MIC

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A microdilution protocol was applied to determine the minimum inhibitory concentration (MIC) of the synthesized nanosilver. Briefly, binary serial dilutions from the nanopowder were prepared in Luria–Bertani (LB) broth (Thermo Fischer Scientific) using sterile 96-well Nunc plates (Thermo Fischer Scientific) and then inoculated with microbial suspensions of 10⁶ (colony-forming units) CFU/mL (in phosphate-buffered saline (PBS), Sigma/Merck). After standard incubation for 24 h, the MIC values were macroscopically evaluated (through naked-eye analysis) and spectrophotometrically determined (as the lowest concentration of AgNPs that inhibited microbial growth) [142 (link),143 (link)].

Constantinescu S., Niculescu A.G., Hudiță A., Grumezescu V., Rădulescu D., Bîrcă A.C., Irimiciuc S.A., Gherasim O., Holban A.M., Gălățeanu B., Oprea O.C., Ficai A., Vasile B.Ș., Grumezescu A.M., Bolocan A, & Rădulescu R. (2023). Silver/Graphene Oxide Nanostructured Coatings for Modulating the Microbial Susceptibility of Fixation Devices Used in Knee Surgery. International Journal of Molecular Sciences, 25(1), 246.

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Maintenance of Human iPSCs in Hypoxic Conditions

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The successfully reprogrammed hiPSCs were incubated in hypoxic conditions (5% CO2, 5% O₂) at 37 °C and maintained in StemFlex™ media (Gibco) on 6-well NUNC™ plates (ThermoFisher) coated with Geltrex™ (ThermoFisher). Cells were passaged (at a ratio between 1:6 and 1:18) upon reaching 60–70% confluency. During passage, cells were washed with room temperature Hank’s Balanced Salt Solution (HBSS) and incubated at 37 °C with Versene (EDTA) solution (Lonza) for 3–5 min, then replated in new Geltrex™-coated NUNC™ plates.

Bhat A., Irizar H., Couch A.C., Raval P., Duarte R.R., Dutan Polit L., Hanger B., Powell T., Deans P.J., Shum C., Nagy R., McAlonan G., Iyegbe C.O., Price J., Bramon E., Bhattacharyya S., Vernon A.C, & Srivastava D.P. (2022). Attenuated transcriptional response to pro-inflammatory cytokines in schizophrenia hiPSC-derived neural progenitor cells. Brain, Behavior, and Immunity, 105, 82-97.

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Isoeugenol MIC Determination for E. coli and L. innocua

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Minimum inhibition concentration values were determined using absorbance measurements of serial dilutions of isoeugenol into TSB in concentrations ranging from 0 to 0.6 mg/mL and 0 to 1.5 mg/mL with or without 5 × 10⁵ CFU/mL of E. coli and L. innocua, respectively. Triplicate cell cultures were prepared in MES-buffer with final cell densities of 10⁷ CFU/mL. Microtiter plates (No. 260887, NUNC plates, Thermo Scientific) with 96-wells were prepared by adding 100 μL TSB in all wells, then adding isoeugenol in a 1.5-serial dilution series to a final concentration ranging either between 0 to 1.2 or 0 to 3 mg/mL. Then 90 μL TSB and 10 μL cell culture were added. Cell-free controls with isoeugenol, and isoeugenol-free controls with cells were prepared in triplicates. The OD₆₂₀ was monitored sequentially every 20 min, for 24 h at 25°C using a plate reader (Sunrise, Tecan, Männedorf, Switzerland). The OD₆₂₀ from cell-free wells containing isoeugenol concentrations were subtracted from sample wells in order to remove background absorbance from isoeugenol. The MIC value was defined as the first concentration resulting in an average increase of OD₆₂₀ ≤ 5% of the growth controls OD₆₂₀ values.

Hyldgaard M., Mygind T., Piotrowska R., Foss M, & Meyer R.L. (2015). Isoeugenol has a non-disruptive detergent-like mechanism of action. Frontiers in Microbiology, 6, 754.

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Quantifying Bacterial Adhesion Assay

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Bacterial adhesion was analyzed in 96-well NUNC plates (Thermo Fisher) coated with dilution buffer (15 mM Na₂HCO₃, 35 mM NaHCO₃, 3.2 mM NaN₃) and incubated overnight at 4°C. Plates were then washed 3 times with wash buffer (150 mM NaCl and 0.01% Tween-20), blocked with 1% BSA, 0.05% Tween-20 solution in PBS for 1 hour at 37°C, and subsequently washed 3 times with wash buffer. S. aureus was added and incubated for 2 hours at 37°C. Plates were washed 3 times, fixed for 30 minutes with 25% formaldehyde solution, washed, and stained with 0.1% crystal violet. Bound crystal violet was solubilized in 10% acetic acid and quantified in a plate reader at 570 nm.

Negrón O., Weggeman M., Grimbergen J., Clark E.G., Abrahams S., Hur W.S., Koopman J, & Flick M.J. (2023). Fibrinogen γ′ promotes host survival during Staphylococcus aureus septicemia in mice. Journal of thrombosis and haemostasis : JTH, 21(8), 2277-2290.

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Measurement of Intracellular ROS Levels

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CaSki and SiHa cells (2 × 10⁵) were seeded in 60-mm Petri dishes. After the treatment with 16 μM B5 for 1–4 h, cells were incubated with 10 μM DCFH-DA for 15 min in the dark, washed three times with ice-cold PBS, and seeded in 96-well NUNC plates (Thermo, USA) at the density of 5 × 10³ per well; DCFH-DA fluorescence was measured at 525 nm using a microplate reader (Tecan F500, Switzerland).

Shao F.Y., Du Z.Y., Ma D.L., Chen W.B., Fu W.Y., Ruan B.B., Rui W., Zhang J.X., Wang S., Wong N.S., Xiao H., Li M.M., Liu X., Liu Q.Y., Zhou X.D., Yan H.Z., Wang Y.F., Chen C.Y., Liu Z, & Chen H.Y. (2015). B5, a thioredoxin reductase inhibitor, induces apoptosis in human cervical cancer cells by suppressing the thioredoxin system, disrupting mitochondrion-dependent pathways and triggering autophagy. Oncotarget, 6(31), 30939-30956.

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