Transmission electron microscopy was performed as described previously with some modifications [38 (link)]. E. faecalis cells mock treated or induced with 100 μM AI-2 for 6 hours were resuspended in 1% (v/v) glutaraldehyde (Sigma). Copper grids (mesh Formvar-carbon coated) were incubated for 30 mins with carbon side on a drop of E. faecalis cells +/- AI-2. Grids were washed 3 times for 5 mins with drops of 0.02 M glycine in PBS followed by 5 consecutive washes in ultra-pure H2O. Bacteria and phages were stained by incubation of the grids for 5 mins on drops containing 1.8% (w/v) methylcellulose (25 centipoises; Sigma-Aldrich) and 0.4% (w/v) uranyl acetate (pH = 4) and subsequently air dried for 10 min. Grids were examined using a Jeol 1010 transmission electron microscope (Jeol Europe, The Netherlands).
1010 transmission electron microscope
Manufactured by JEOL
Sourced in Japan, United States, United Kingdom
The JEOL 1010 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of specimens. It operates at an accelerating voltage of 100 kV and provides a magnification range from 50x to 1,000,000x. The JEOL 1010 TEM is capable of producing detailed images of the internal structure and composition of various materials at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
Ultracut e ultramicrotome, by Reichert Technologies (11 mentions)
Milli q water, by Merck Group (4 mentions)
Uct ultramicrotome, by Leica (3 mentions)
Reynold s lead citrate, by Merck Group (3 mentions)
Orius ccd camera, by Ametek (3 mentions)
Millipore gradient a10, by Merck Group (3 mentions)
Thermomixer, by Eppendorf (2 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Formvar carbon coated copper grid, by Electron Microscopy Sciences (2 mentions)
Ultracut uct ultramicrotome, by Leica (2 mentions)
V 630, by Jasco (2 mentions)
Epon resin, by Merck Group (2 mentions)
1010 tem, by JEOL (2 mentions)
Nanogold 1.4 nm, by Nanoprobes (2 mentions)
Methylcellulose, by Merck Group (1 mentions)
90 plus particle size analyzer, by Brookhaven Instruments (1 mentions)
Jsm 5900lv, by JEOL (1 mentions)
Agilent 8453 uv visible spectrometer, by Agilent Technologies (1 mentions)
Model 421, by Lake Shore Cryotronics (1 mentions)
Hq silver enhancement kit, by Nanoprobes (1 mentions)
105 protocols using 1010 transmission electron microscope
1
Transmission Electron Microscopy of E. faecalis
2
Physicochemical Characterization of Magnetic Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Structural conformation of synthesized MNPs was verified using Bruker GADDS/D8 X-ray diffraction system with Apex Smart CCD Detector and Mo direct-drive rotating anode (50 kV; 20 mA). Diffraction patterns were analyzed and indexed using ICDD PDF 2015 database and Match software. Further, to confirm the elemental composition of MNPs, energy dispersive spectroscopy (EDS) was conducted in scanning electron microscopy (JEOL JSM 5900LV) at 15 kV and working distance of 10 mm.
The hydrodynamic radius and size distribution of MNPs were analyzed using dynamic laser scattering (DLS) (90 Plus particle size analyzer, Brookhaven Instruments, USA) at room temperature. Further, to examine the original crystal size, transmission electron microscopy (TEM) analysis was performed with the JEOL 1010 Transmission Electron microscope operated at 100 kV. The magnetization curve of MNPs was measured using vibrating sample magnetometer (VSM-3, Toei Kogyo, Tokyo, Japan) equipped with an electromagnet (TEM-WFR7, Toei Kogyo, Tokyo, Japan) and a gaussmeter (Model 421, Lake Shore Cryotronics, Inc.). The measurement was conducted at room temperature with a maximum field of 780 kA/m.
The Agilent 8453 UV-Visible Spectrometer with Quartz-1 cm path length was used for evaluating absorbance of MNPs from 200 to 1000 nm wavelength.
The hydrodynamic radius and size distribution of MNPs were analyzed using dynamic laser scattering (DLS) (90 Plus particle size analyzer, Brookhaven Instruments, USA) at room temperature. Further, to examine the original crystal size, transmission electron microscopy (TEM) analysis was performed with the JEOL 1010 Transmission Electron microscope operated at 100 kV. The magnetization curve of MNPs was measured using vibrating sample magnetometer (VSM-3, Toei Kogyo, Tokyo, Japan) equipped with an electromagnet (TEM-WFR7, Toei Kogyo, Tokyo, Japan) and a gaussmeter (Model 421, Lake Shore Cryotronics, Inc.). The measurement was conducted at room temperature with a maximum field of 780 kA/m.
The Agilent 8453 UV-Visible Spectrometer with Quartz-1 cm path length was used for evaluating absorbance of MNPs from 200 to 1000 nm wavelength.
3
Negative Staining of Vesicles for TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electron microscopy was performed on a Jeol 1010 transmission electron microscope (Jeol, Warwickshire, Welwyn Garden, UK). Uranyl acetate (0.5%) was used to negatively stain the vesicles in the samples, as described previously [11 ].
4
Transmission Electron Microscopy of Grayling Testes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To produce tissue specimens for TEM observation of the testicular structure of grayling, the gonads were minced and fixed in 2.5% paraformaldehyde/glutaraldehyde in 0.1 M phosphate buffer solution (pH 7.4) at 4°C for 2 h. Subsequently, the samples were washed thrice with the same buffer solution and postfixed in 2% osmium tetroxide solution in the buffer solution at 4°C for 1 h. The samples were then dehydrated in ascending acetone concentrations (30, 50, 70, 90, 95, and 100%) for 15 min each and embedded in resin (Epon 812, Germany). Ultrathin sections (0.07–0.08 μm) were sliced using a UCT ultramicrotome (Leica Microsystems, Wetzlar, Germany). These were mounted on copper grids, double-stained with uranyl acetate (saturated in 100% alcohol) for 30 min, and counterstained with 1% lead citrate for 20 min. A 1010 transmission electron microscope (JEOL, Tokyo, Japan) operated at 80 kV was used to examine the samples (15 (link), 26 (link)).
5
Exosome Visualization by Electron Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated exosomes were fixed in 4% paraformaldehyde, adsorbed in formvar-coated cooper grids and negatively stained with 2% uranyl acetate. Grids were air dried and observed in a JEOL-1010 Transmission Electron Microscope9 (link).
6
Immunogold Labeling of Ciliary TβRI
7
Immunogold Labeling of Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated EVs were fixed in 2% paraformaldehyde, adsorbed in formvar-coated nickel grids for 20 min and negative stained with 4% uranyl oxalate. Grids were air dried and observed in a JEOL-1010 Transmission Electron Microscope at 80 kV.
For the immunogold labeling, grids were blocked with 1% BSA for 10 min, followed by anti-human REG3β antibody incubation for 1 h and 12 nm gold-conjugated goat anti-rabbit IgG secondary antibody incubation (Jackson Laboratories) for 30 min. Then, samples were further fixed with 1% glutaraldehyde, counterstained with 4% uranyl oxalate and embedded with a mixture of 2% methyl cellulose - 4% uranyl acetate.
For the immunogold labeling, grids were blocked with 1% BSA for 10 min, followed by anti-human REG3β antibody incubation for 1 h and 12 nm gold-conjugated goat anti-rabbit IgG secondary antibody incubation (Jackson Laboratories) for 30 min. Then, samples were further fixed with 1% glutaraldehyde, counterstained with 4% uranyl oxalate and embedded with a mixture of 2% methyl cellulose - 4% uranyl acetate.
8
Fibrillization Kinetics of hnRNPA1 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hnRNPA1 (5 μM) fibrillization (100 μL reaction) was initiated by addition of 2 μg of TEV protease in A1 assembly buffer (40 mM HEPES-NaOH at pH 7.4, 150 mM KCl, 5% glycerol, 1 mM DTT, and 20 mM glutathione). hnRNPA1 fibrillization reactions were incubated at 25°C for 24 hours with agitation at 1200 rpm in an Eppendorf Thermomixer, at which time fibrillization was complete with approximately 100% of the hnRNPA1 in the aggregated state. For sedimentation analysis, at indicated times, fibrillization reactions were centrifuged at 16,100g for 10 minutes at 4°C. Supernatant and pellet fractions were resolved by SDS-PAGE and stained with Coomassie Brilliant Blue, and the relative amount in each fraction was determined by densitometry in ImageJ (NIH). Fibrillization assays were performed in triplicate. For electron microscopy, fibrillization reactions (10 μL) were adsorbed onto glow-discharged 300-mesh Formvar/carbon-coated copper grids (Electron Microscopy Sciences) and stained with 2% (w/v) aqueous uranyl acetate. Excess liquid was removed, and grids were air-dried. Samples were viewed using a JEOL 1010 transmission electron microscope.
9
Transmission Electron Microscopy of Viral Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Confluent cells in 6 well plates were inoculated with PR8 virus for 2 h at 37 °C, and then treated with 250 μg/ml KW or 30 μM Zanamivir in post-adsorption medium for 24 h at 37 °C. Cells were scraped off and centrifuged at 1600 × g for 5 min. Medium was discarded and cells were incubated with ice-cold fixative (2.5% glutaraldehyde in 0.1 M PBS buffer, pH 7.4) for 50 min, with gentle agitation. Cells were pelleted by centrifugation at 20,000 × g for 2 min at RT. Cell pellets were resuspended in 0.5 ml fixative solution then rinsed in 0.5 M cacodylate buffer twice for 10 min and post-fixed with 2% osmium tetroxide for 2 h. The fixed cells were washed with water twice for 10 min, dehydrated with increasing concentrations of ethanol from 50 to 100% and embedded in spurr resin. Thin (70–80 nm) sections were cut on an ultramicrotome and counter stained with uranyl acetate and lead citrate. The sections were viewed and photographed on a JEOL 1010 transmission electron microscope.
10
Visualizing Streptomyces Spore Chains by TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For visualizing the spore chains of Streptomyces, small blocks were cut from SFM plates harvested in confluent cultures and processed for TEM essentially as described (Piette et al. 2005 (link)). The mycelium was washed with 1 × PBS before fixation with 1.5% glutaraldehyde, and blocks were then post-fixed with 1% osmium tetroxide for 30 min. The samples were dehydrated by passing them through an ethanol gradient and placed in propylene oxide for 15 min followed by incubation in a mixture of Epon and propylene oxide (1:1) and pure Epon (each step 45 min). Finally, the samples were embedded in Epon and sectioned into 70 nm slices, which were placed on 200-mesh copper grids. Samples were stained using uranyl-430 acetate (2%) and lead citrate (0.4%), if necessary, and imaged at 70 kV in a Jeol 1010 transmission electron microscope.
Three biological replicates; 3 plates/mutant, and three methodological replicates; 3 blocks per plate were used for SEM experiments.
Three biological replicates; 3 plates/mutant, and three methodological replicates; 3 blocks per plate were used for SEM experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!