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Anti tubulin primary antibody

Manufactured by Merck Group
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The Anti-tubulin primary antibody is a lab equipment product that binds to tubulin, a key structural protein found in the cytoskeleton of cells. This antibody can be used to detect and study the distribution and dynamics of tubulin in various cell types and biological samples.

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Lab products found in correlation

P erk1 2, by Cell Signaling Technology (3 mentions) Phospho erk1 2 p erk1 2, by Cell Signaling Technology (2 mentions) Anti human igg fc antibody coupled to hrp, by Abcam (2 mentions) Anti gst sc 138, by Santa Cruz Biotechnology (1 mentions) Ni nta agarose, by Thermo Fisher Scientific (1 mentions) Glutathione sepharose, by GE Healthcare (1 mentions) Prestoblue cell viability reagent, by Thermo Fisher Scientific (1 mentions) Protein a sepharose, by GE Healthcare (1 mentions) Anti gst antibody, by Merck Group (1 mentions) Heparin sepharose, by GE Healthcare (1 mentions) P fgfr, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Piperidine, by Iris Biotech (1 mentions) Heparin sepharose resin, by GE Healthcare (1 mentions) Anti fgfr1 fgfr1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Heparin, by Merck Group (1 mentions) Anti poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions) Chemidoc station, by Bio-Rad (1 mentions) Pd173074, by Merck Group (1 mentions)

Spelling variants of this product

Anti-tubulin (542 citations) Anti-tubulin antibody (103 citations) Anti-α-tubulin primary antibody (7 citations) Mouse anti-Tubulin primary antibody (5 citations) Primary monoclonal anti-α-tubulin antibody (4 citations) Mouse anti-alpha tubulin primary antibody (4 citations) Mouse-anti-α tubulin primary antibody (4 citations) Mouse anti–β-tubulin primary antibody (2 citations) -anti-βIII-tubulin primary antibody (2 citations)

6 protocols using anti tubulin primary antibody

1

Western blot analysis of FGFR1 and ERK

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The primary antibodies directed against FGFR1 (#9740), phospho-FGFR (pFGFR; #3476), ERK1/2 (#9102) and phospho-ERK1/2 (pERK1/2; #9101) were from Cell Signaling (Danvers, MA, USA). Anti-tubulin primary antibody (#T6557) was from Sigma-Aldrich (St Louis, MO, USA), and anti-FGF1 (sc-55520), anti-FGF2 (sc-365106) and anti-GST (sc-138) primary antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies coupled to HRP were from Jackson Immuno-Research Laboratories (Cambridge, UK).
Poźniak M., Porębska N., Jastrzębski K., Krzyścik M.A., Kucińska M., Zarzycka W., Barbach A., Zakrzewska M., Otlewski J., Miączyńska M, & Opaliński Ł. (2021). Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors. Journal of Biomedical Science, 28, 69.
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2

Western Blot Immunodetection of ERK1/2 Activation

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The primary antibodies directed against ERK1/2 (#9102) and phospho-ERK1/2 (pERK1/2; #9101) were from Cell Signaling (Danvers, MA, USA). Anti-tubulin primary antibody (#T6557) was from Sigma-Aldrich (St. Louis, MO, USA), anti-FGF1 primary antibody (sc-55520) was from Santa Cruz Biotechnology (Dallas, TX, USA) and anti-human IgG (Fc) antibody coupled to HRP (#4-10-20) was from KPL (Gaithersburg, MA, USA). Secondary antibodies coupled to HRP were from Jackson Immuno-Research Laboratories (Cambridge, UK).
Poźniak M., Zarzycka W., Porębska N., Knapik A., Marczakiewicz-Perera P., Zakrzewska M., Otlewski J, & Opaliński Ł. (2021). FGF1 Fusions with the Fc Fragment of IgG1 for the Assembly of GFPpolygons-Mediated Multivalent Complexes Recognizing FGFRs. Biomolecules, 11(8), 1088.
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3

FGFR and ERK1/2 Signaling Pathway Antibodies

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The primary antibodies directed against FGFR1 (#9740), FGFR2 (#11835), FGFR3 (#4574), phospho-FGFR1 (pFGFR1; #3476), ERK1/2 (#9102), and phospho-ERK1/2 (pERK1/2; #9101) were from Cell Signaling (Danvers, MA, USA). The anti-tubulin primary antibody (#T6557) and the anti-GST antibody (#G1160) were from Sigma-Aldrich (St. Louis, MO, USA). Anti-human IgG (Fc) antibody coupled to HRP (#ab97225) was from Abcam (Cambridge, UK). The primary antibodies directed against FGFR4 (#sc-136988) and the anti-His-Tag primary antibodies (#sc-8036) were from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies were obtained from Jackson Immuno-Research Laboratories (Cambridge, UK). Protein A Sepharose, Glutathione Sepharose, and Heparin Sepharose resins were from GE Healthcare (Chicago, IL, USA). Ni–NTA agarose and PrestoBlue™ Cell Viability Reagent (Thermo Fisher Scientific, Waltham, MA, USA). α-Lactose Separopore resin was from bioWORLD (Irving, TX, USA).
Zukowska D., Gedaj A., Porebska N., Pozniak M., Krzyscik M., Czyrek A., Krowarsch D., Zakrzewska M., Otlewski J, & Opalinski L. (2023). Receptor clustering by a precise set of extracellular galectins initiates FGFR signaling. Cellular and Molecular Life Sciences, 80(4), 113.
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4

FGFR1-Mediated Signaling Pathway Analysis

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The primary
antibodies directed against FGFR1 (#9740), phospho-FGFR (p-FGFR, #3476),
ERK1/2 (#9102), and phospho-ERK1/2 (p-ERK1/2, #9101) were from Cell
Signaling (Danvers, MA, USA). The antitubulin primary antibody (#T6557)
was from Sigma-Aldrich (St. Louis, MO, USA). The anti-FGF1 primary
antibody (#sc-55520) was from Santa Cruz Biotechnology (Dallas, TX,
USA). HRP-conjugated secondary antibodies were obtained from Jackson
Immuno-Research Laboratories (Cambridge, UK).
Reagents used
for the solid-phase peptide synthesis were as follows: amino Fmoc-Gly-OH,
Fmoc-l-Cys(StBu)–OH, Fmoc-O2Oc-O2Oc–OH; COMU
(1-[1-(cyano-2-ethoxy-2-oxoethylideneaminooxy)-dimethylamino-morpholino]uroniumhexafluorophosphate),
EDT (ethane-1,2-dithiol), piperidine, TIS (triisopropylsilane), DIPEA
(N,N-diisopropylethylamine), DMF
(N,N-dimethylformamide), DCM (dichloromethane),
and TFA (trifluoroacetic acid) were purchased from Iris Biotech GmbH
(Marktredwitz, Germany). HPLC-pure acetonitrile and Et2O (diethyl ether) were from Avantor (Gliwice, Poland). TentaGel S
RAM resin (particle size, 90 μm; loading, 0.21 mmol/g) was from
Rapp Polymere GmbH (Tübingen, Germany). The cytotoxic agents,
MMAE (monomethyl auristatin E) and MC-vc-PAB–MMAE, were from
MedChemExpress (Monmouth Junction, NJ, USA). A Synergi 4 μm
Fusion-RP 80 Å 250 × 10 mm2 LC column was from
Phenomenex, Inc.
Porębska N., Knapik A., Poźniak M., Krzyścik M.A., Zakrzewska M., Otlewski J, & Opaliński Ł. (2021). Intrinsically Fluorescent Oligomeric Cytotoxic Conjugates Toxic for FGFR1-Overproducing Cancers. Biomacromolecules, 22(12), 5349-5362.
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5

Phospho-FGFR Signaling Pathway Analysis

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The primary antibodies: anti-phospho-FGFR (Tyr653/Tyr654) (p-FGFR) (#06-1433), anti-FGFR1 (FGFR1) (#9740), anti-phospho-p44/42 (Thr202/Tyr204) MAP kinase antibody (p-Erk1/2) (#9101), anti-p44/42 MAP kinase antibody (Erk1/2) (#9102), anti-caspase 3 (caspase 3) (#9662) and anti-poly [ADP-ribose] polymerase (PARP) (#9542) were from Cell Signaling Technology. The primary antibodies anti-FHF1 antibody (#PA5-67182) was from Thermo Fisher Scientific. Anti-tubulin primary antibody (#T6557) were from Sigma-Aldrich. Anti-human IgG (Fc) antibody coupled to HRP (#ab97225) was from Abcam. Horseradish peroxidase-conjugated secondary antibodies were from Jackson ImmunoResearch and chemiluminescent substrate was used for their visualization in the ChemiDoc station (BioRad). heparin Sepharose resin was from GE Healthcare. Geneticin (G-418) was from BioShop. PD173074 and heparin were from Sigma-Aldrich.
Sochacka M., Opalinski L., Szymczyk J., Zimoch M.B., Czyrek A., Krowarsch D., Otlewski J, & Zakrzewska M. (2020). FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner. Cell Communication and Signaling : CCS, 18, 69.
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6

Immunoblotting for FGFR1 and ERK Signaling

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The primary antibodies directed against FGFR1 (#9740), phospho-FGFR (pFGFR; #3476), ERK1/2 (#9102) and phospho-ERK1/2 (pERK1/2; #9101) were from Cell Signaling (Danvers, MA, USA). Anti-tubulin primary antibody (#T6557) was from Sigma-Aldrich (St Louis, MO, USA) and anti-human IgG (Fc) antibody coupled to HRP (#4-10-20) was from KPL (Gaithersburg, MA, USA). Secondary antibodies coupled to HRP were from Jackson Immuno-Research Laboratories (Cambridge, UK).
Poźniak M., Porębska N., Krzyścik M.A., Sokołowska-Wędzina A., Jastrzębski K., Sochacka M., Szymczyk J., Zakrzewska M., Otlewski J, & Opaliński Ł. (2021). The cytotoxic conjugate of highly internalizing tetravalent antibody for targeting FGFR1-overproducing cancer cells. Molecular Medicine, 27, 46.
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