Ldh cytotoxicity assay kit

Cell viability was determined by the CCK-8 assay, according to the manufacturer's instructions. PC12 cells were cultured in 6-well plates and pretreated with various conditions (HG, HH/R, and HH/R-H) as described previously, following which 10 μL CCK-8 (C0037, Beyotime, China) was added and cells were incubated for 4 hours, and the absorbance at 450 nm was measured using a microplate reader. The mean optical density (OD) of 6 wells in each group was used to calculate the percentage of cell viability. The supernatant was collected to measure the effluent LDH after PC12 cells performed above pretreatment. LDH release was measured by LDH Cytotoxicity Assay Kit (Nanjing Jiancheng Bio-Engineering Institute, China).

Cell viability was determined by using a cell counting kit-8 (CCK-8) assay, according to the manufacturer's instructions. HK-2 cells (1 × 10⁵ cells/well) were plated into 96-well plates and pretreated with various conditions (NG, HG, NH/R, HH/R, HH/R-siRNA, HH/R-scrambled siRNA, and HH/R-RES) as described, following which, 10 µL CCK-8 (Beyotime: C0037, China) was added and cells were incubated for 4 hours, and the absorbance was measured at 450 nm with an ELISA assay plate reader. LDH content was measured by LDH Cytotoxicity Assay Kit (Jiancheng Biotech, Nanjing, China).

Immediately following OGD/R, the culture supernatants were collected and, subsequently, the level of lactate dehydrogenase (LDH) was detected using the LDH Cytotoxicity Assay Kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) according to the manufacturer’s protocol.

The cycotoxicity of furanodienone on human colon cells was assessed with LDH cytotoxicity Assay Kit (Jiancheng, Nanjing, China) according to the manufacture’s instructions.

After the treatment period, the RAW264.7 cells culture medium was collected and assayed for LDH activity. The LDH release assay was detected using an LDH cytotoxicity assay kit (Nanjing Jiancheng Institute of Bioengineering, Nanjing, China), according to the manufacturer’s protocol.

The lactate dehydrogenase (LDH) assay was performed using the LDH Cytotoxicity Assay Kit (A020-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s protocol. The amount of LDH released by cells can be used as an index of cellular damage. The kit contains WST (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) reagent for detection of LDH released from the damaged cells. Reacting with WST, LDH oxidizes lactate to generate NADH, which can be quantified at 450 nm optical density by a ELIASA (Infinite 200 PRO, Tecan).