Penicillin

Manufactured by Sino Biological

Penicillin is a class of antibiotics derived from the Penicillium fungus. It is used in the laboratory for the cultivation and identification of microorganisms.

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Lab products found in correlation

Streptomycin, by Sino Biological (2 mentions) Gm csf, by Sino Biological (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by Sino Biological (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Hek293ft cells, by Thermo Fisher Scientific (1 mentions) Smm 293 ti, by Sino Biological (1 mentions) Penicillin, by Solarbio (1 mentions) Streptomycin, by Solarbio (1 mentions) Ficoll paque, by GE Healthcare (1 mentions) Fetal bovine serum (fbs), by STEMCELL (1 mentions) M csf, by Sino Biological (1 mentions) Rpmi 1640 medium, by STEMCELL (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Macs microbead, by Miltenyi Biotec (1 mentions) Macrophage colony stimulating factor, by R&D Systems (1 mentions)

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Penicillin-streptomycin (5 citations)

3 protocols using penicillin

Culturing and Characterizing Mouse Breast Carcinoma and Reporter Cells

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4T1 mouse breast carcinoma
was purchased from ATCC and cultured in complete Dulbecco’s
modified Eagle’s medium (DMEM, Gibco) supplemented with 10%
fetal bovine serum (FBS; Gibco), 100 U/mL penicillin (HyClone), and
100 μg/mL streptomycin (HyClone). HEK-Blue hTLR7 reporter cells
were purchased from InvivoGen. The cell lines were maintained and
subcultured in growth medium (DMEM, 10% FBS, 50 U/mL penicillin, 50
μg/mL streptomycin, 100 mg/mL Normocin, 2 mM l-glutamine)
supplemented with 10 μg/mL of blasticidin and 100 μg/mL
of Zeocin. Bone marrow-derived dendritic cells (BMDCs) were harvested
from 8-week-old Balb/c mice. BMDCs were cultured in BMDC primary media:
RPMI 1640 (HyClone), 10% FBS, 100 U/mL penicillin, 100 μg/mL
streptomycin, 20 ng/mL GM-CSF (Sino Biological), and 10 ng/mL IL-4
(Sino Biological). All cell lines and assay cultures were maintained
at 37 °C and 5% CO₂. Cell lines were tested regularly
for mycoplasma contamination, and none used tested positive at any
point.

Tang L., Cai D., Qin M., Lu S., Hu M.H., Ruan S., Jin G, & Wang Z. (2019). Oxaliplatin-Based Platinum(IV) Prodrug Bearing Toll-like Receptor 7 Agonist for Enhanced Immunochemotherapy. ACS Omega, 5(1), 726-734.

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Establishing and Purifying HEK293FT Cell Line

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HEK293FT cells (Invitrogen, R70007) were cultured in DMEM medium (Hyclone, SH30243.01B) supplemented with 10% fetal bovine serum (FBS) (Biological Industries, 04–001–1 A), 1x glutaMAX (Gibco, 35050-061), 100 U/ml penicillin and 10 mg/ml streptomycin (Solarbio, P1400), at 37 °C in a 5.5% CO₂ humidified atmosphere for stable cell line establishment. When establishment completed, cells were transferred to flanking bottles in SMM 293-TI (Sino Biological inc, M293TI-1), containing 1% FBS, 1x glutaMAX, 100 U/ml penicillin and 10 mg/ml streptomycin for purification. Cell lines were not authenticated or tested for mycoplasma contamination.

Liang X., Zuo M.Q., Zhang Y., Li N., Ma C., Dong M.Q, & Gao N. (2020). Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. Nature Communications, 11, 3542.

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Isolation and Culture of Human and Mouse Macrophages

Cited 1 time

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Peripheral blood mononuclear cells (PBMCs) were isolated from human blood using Ficoll-Paque (GE Healthcare, Chicago, IL, USA). Human monocyte-derived macrophages were purified by positive selection of CD14 and CD16 cells from the PBMCs using MACS Microbeads from Miltenyi Biotec (Leiden, Netherlands), following the manufacturer's protocol. The isolated monocytes were then induced with 20 ng/mL of macrophage colony-stimulating factor (R&D Systems, Minneapolis, USA) in RPMI-1640 medium supplemented with 10% fetal bovine serum (STEMCELL Technologies), 100 U/mL of penicillin/streptomycin (Thermo Fisher Scientific), 10 mM Glutamax, and 10 mM pyruvate. Mouse alveolar macrophages (AMs) and bone marrow-derived macrophages (BMDMs) were isolated from control mice as previously describe [39 (link), 40 (link)] and cultured in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum and 2 mM glutamine with penicillin (100 U/mL)/streptomycin (100 mg/mL) and M-CSF (10 ng/mL, Sino Biological, 51112-M08H). The cells were grown in 96-well plates (200 μL final volume; Corning Inc., Corning, NY, USA) and incubated at 37 °C in a humidified incubator with 5% CO₂.

Su H., Weng S., Luo L., Sun Q., Lin T., Ma H., He Y., Wu J., Wang H., Zhang W, & Xu Y. (2024). Mycobacterium tuberculosis hijacks host macrophages-derived interleukin 16 to block phagolysosome maturation for enhancing intracellular growth. Emerging Microbes & Infections, 13(1), 2322663.

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